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Literature DB >> 8760904

A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

Abstract

We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.

Entities: Gene Species

Mesh：

Substances：

Year: 1996 PMID： 8760904 PMCID： PMC146033 DOI： 10.1093/nar/24.15.3118

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

11 in total

1. Ligand-regulated site-specific recombination.

Authors: C Logie; A F Stewart
Journal: Proc Natl Acad Sci U S A Date: 1995-06-20 Impact factor: 11.205

2. New method for generating deletions and gene replacements in Escherichia coli.

Authors: C M Hamilton; M Aldea; B K Washburn; P Babitzke; S R Kushner
Journal: J Bacteriol Date: 1989-09 Impact factor: 3.490

3. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome.

Authors: K G Golic; S Lindquist
Journal: Cell Date: 1989-11-03 Impact factor: 41.582

4. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.

Authors: M Jayaram
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

Review 5. Genome engineering: the new mouse genetics.

Authors: J Rossant; A Nagy
Journal: Nat Med Date: 1995-06 Impact factor: 53.440

6. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination.

Authors: A J Smith; M A De Sousa; B Kwabi-Addo; A Heppell-Parton; H Impey; P Rabbitts
Journal: Nat Genet Date: 1995-04 Impact factor: 38.330

7. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting.

Authors: H Gu; Y R Zou; K Rajewsky
Journal: Cell Date: 1993-06-18 Impact factor: 41.582

8. Inducible gene targeting in mice.

Authors: R Kühn; F Schwenk; M Aguet; K Rajewsky
Journal: Science Date: 1995-09-08 Impact factor: 47.728

Review 9. Site-specific recombinases: tools for genome engineering.

Authors: N J Kilby; M R Snaith; J A Murray
Journal: Trends Genet Date: 1993-12 Impact factor: 11.639

10. Chromosome engineering in mice.

Authors: R Ramírez-Solis; P Liu; A Bradley
Journal: Nature Date: 1995-12-14 Impact factor: 49.962

38 in total

1. Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.

Authors: H Vilen; S Eerikäinen; J Tornberg; M S Airaksinen; H Savilahti
Journal: Transgenic Res Date: 2001 Impact factor: 2.788

2. RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.

Authors: J P Muyrers; Y Zhang; F Buchholz; A F Stewart
Journal: Genes Dev Date: 2000-08-01 Impact factor: 11.361

3. Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells.

Authors: Susanne Andreas; Frieder Schwenk; Birgit Küter-Luks; Nicole Faust; Ralf Kühn
Journal: Nucleic Acids Res Date: 2002-06-01 Impact factor: 16.971

A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

1. Ligand-regulated site-specific recombination.

2. New method for generating deletions and gene replacements in Escherichia coli.

3. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome.

4. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.

Review 5. Genome engineering: the new mouse genetics.

6. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination.

7. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting.

8. Inducible gene targeting in mice.

Review 9. Site-specific recombinases: tools for genome engineering.

10. Chromosome engineering in mice.

1. Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.

2. RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.

3. Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells.

4. A genetic screen identifies novel non-compatible loxP sites.

5. Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method.

6. Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference.

7. Constitutive turnover of cyclin E by Cul3 maintains quiescence.

8. Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci.

9. Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination.

10. Intergenic transcription through a polycomb group response element counteracts silencing.