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Literature DB >> 3898075

Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.

Abstract

The 2-mum circle DNA of yeast encodes a site-specific recombination system (FLP recombination). The recombination region had been mapped earlier to a 65-base-pair (bp) segment within the 599-bp-long inverted repeats of the molecule. I have shown that the "minimal" FLP substrate resides in a 13-bp dyad symmetry plus an 8-bp core located within the 65-bp recombination region. Further, as determined by different in vivo assays, sequences extraneous to the minimal FLP site and the 65-bp recombination region can affect the efficiency of the recombination reaction.

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Year: 1985 PMID： 3898075 PMCID： PMC390656 DOI： 10.1073/pnas.82.17.5875

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Site specific recombination involved in the generation of small plasmids.

Authors: E Ohtsubo; M Rosenbloom; H Schrempf; W Goebel; J Rosen
Journal: Mol Gen Genet Date: 1978-02-16

2. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

Authors: J R Broach; J N Strathern; J B Hicks
Journal: Gene Date: 1979-12 Impact factor: 3.688

3. Transposon-mediated site-specific recombination: a defined in vitro system.

Authors: R R Reed
Journal: Cell Date: 1981-09 Impact factor: 41.582

4. Phase variation: evolution of a controlling element.

Authors: M Simon; J Zieg; M Silverman; G Mandel; R Doolittle
Journal: Science Date: 1980-09-19 Impact factor: 47.728

5. Recombination within the yeast plasmid 2mu circle is site-specific.

Authors: J R Broach; V R Guarascio; M Jayaram
Journal: Cell Date: 1982-05 Impact factor: 41.582

6. Somatic reorganization of immunoglobulin genes during lymphocyte differentiation.

Authors: S Tonegawa; H Sakano; R Make; A Traunecker; G Heinrich; W Roeder; Y Kurosawa
Journal: Cold Spring Harb Symp Quant Biol Date: 1981

7. Replication and recombination functions associated with the yeast plasmid, 2 mu circle.

Authors: J R Broach; J B Hicks
Journal: Cell Date: 1980-09 Impact factor: 41.582

8. Nucleotide sequence of the yeast plasmid.

Authors: J L Hartley; J E Donelson
Journal: Nature Date: 1980-08-28 Impact factor: 49.962

9. Mechanism of action of the cro protein of bacteriophage lambda.

Authors: A Johnson; B J Meyer; M Ptashne
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

10. Transformation of intact yeast cells treated with alkali cations.

Authors: H Ito; Y Fukuda; K Murata; A Kimura
Journal: J Bacteriol Date: 1983-01 Impact factor: 3.490

28 in total

1. Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells.

Authors: Susanne Andreas; Frieder Schwenk; Birgit Küter-Luks; Nicole Faust; Ralf Kühn
Journal: Nucleic Acids Res Date: 2002-06-01 Impact factor: 16.971

2. Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.

Authors: Matthias Lauth; Fabio Spreafico; Kathrin Dethleffsen; Michael Meyer
Journal: Nucleic Acids Res Date: 2002-11-01 Impact factor: 16.971

10. The minimal duplex DNA sequence required for site-specific recombination promoted by the FLP protein of yeast in vitro.

Authors: G Proteau; D Sidenberg; P Sadowski
Journal: Nucleic Acids Res Date: 1986-06-25 Impact factor: 16.971

Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.

1. Site specific recombination involved in the generation of small plasmids.

2. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

3. Transposon-mediated site-specific recombination: a defined in vitro system.

4. Phase variation: evolution of a controlling element.

5. Recombination within the yeast plasmid 2mu circle is site-specific.

6. Somatic reorganization of immunoglobulin genes during lymphocyte differentiation.

7. Replication and recombination functions associated with the yeast plasmid, 2 mu circle.

8. Nucleotide sequence of the yeast plasmid.

9. Mechanism of action of the cro protein of bacteriophage lambda.

10. Transformation of intact yeast cells treated with alkali cations.

1. Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells.

2. Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.

3. Domain of a yeast site-specific recombinase (Flp) that recognizes its target site.

4. Site-specific genomic targeting in Drosophila.

5. Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells.

6. Synapsis, strand scission, and strand exchange induced by the FLP recombinase: analysis with half-FRT sites.

7. A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

8. FLP-mediated recombination of FRT sites in the maize genome.

9. Activity of the yeast FLP recombinase in Arabidopsis.

10. The minimal duplex DNA sequence required for site-specific recombination promoted by the FLP protein of yeast in vitro.