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Literature DB >> 8760890

Covalent attachment of synthetic DNA to self-assembled monolayer films.

Abstract

The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.

Entities: Chemical Species

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Year: 1996 PMID： 8760890 PMCID： PMC146042 DOI： 10.1093/nar/24.15.3031

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

14 in total

1. Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.

Authors: U Maskos; E M Southern
Journal: Nucleic Acids Res Date: 1992-04-11 Impact factor: 16.971

2. Fabrication of surfaces resistant to protein adsorption and application to two-dimensional protein patterning.

Authors: S K Bhatia; J L Teixeira; M Anderson; L C Shriver-Lake; J M Calvert; J H Georger; J J Hickman; C S Dulcey; P E Schoen; F S Ligler
Journal: Anal Biochem Date: 1993-01 Impact factor: 3.365

3. DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing.

Authors: R Drmanac; S Drmanac; Z Strezoska; T Paunesku; I Labat; M Zeremski; J Snoddy; W K Funkhouser; B Koop; L Hood
Journal: Science Date: 1993-06-11 Impact factor: 47.728

4. Direct detection of nucleic acid hybridization on the surface of a charge coupled device.

Authors: J B Lamture; K L Beattie; B E Burke; M D Eggers; D J Ehrlich; R Fowler; M A Hollis; B B Kosicki; R K Reich; S R Smith
Journal: Nucleic Acids Res Date: 1994-06-11 Impact factor: 16.971

5. Direct measurement of the forces between complementary strands of DNA.

Authors: G U Lee; L A Chrisey; R J Colton
Journal: Science Date: 1994-11-04 Impact factor: 47.728

6. Adhesive electroless metallization of fluoropolymeric substrates.

Authors: T G Vargo; J A Gardella; J M Calvert; M S Chen
Journal: Science Date: 1993-12-10 Impact factor: 47.728

7. Covalent attachment of oligonucleotides to solid supports.

Authors: S S Ghosh; G F Musso
Journal: Nucleic Acids Res Date: 1987-07-10 Impact factor: 16.971

8. Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids.

Authors: E M Southern; S C Case-Green; J K Elder; M Johnson; K U Mir; L Wang; J C Williams
Journal: Nucleic Acids Res Date: 1994-04-25 Impact factor: 16.971

9. Surface determinants of neuronal survival and growth on self-assembled monolayers in culture.

Authors: D A Stenger; C J Pike; J J Hickman; C W Cotman
Journal: Brain Res Date: 1993-12-10 Impact factor: 3.252

Review 10. Using oligonucleotide probe arrays to access genetic diversity.

Authors: R J Lipshutz; D Morris; M Chee; E Hubbell; M J Kozal; N Shah; N Shen; R Yang; S P Fodor
Journal: Biotechniques Date: 1995-09 Impact factor: 1.993

38 in total

1. Dynamic force spectroscopy of single DNA molecules.

Authors: T Strunz; K Oroszlan; R Schäfer; H J Güntherodt
Journal: Proc Natl Acad Sci U S A Date: 1999-09-28 Impact factor: 11.205

2. Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.

Authors: J D Andreadis; L A Chrisey
Journal: Nucleic Acids Res Date: 2000-01-15 Impact factor: 16.971

Covalent attachment of synthetic DNA to self-assembled monolayer films.

1. Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.

2. Fabrication of surfaces resistant to protein adsorption and application to two-dimensional protein patterning.

3. DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing.

4. Direct detection of nucleic acid hybridization on the surface of a charge coupled device.

5. Direct measurement of the forces between complementary strands of DNA.

6. Adhesive electroless metallization of fluoropolymeric substrates.

7. Covalent attachment of oligonucleotides to solid supports.

8. Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids.

9. Surface determinants of neuronal survival and growth on self-assembled monolayers in culture.

Review 10. Using oligonucleotide probe arrays to access genetic diversity.

1. Dynamic force spectroscopy of single DNA molecules.

2. Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.

3. End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads.

4. Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.

5. Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries.

Review 6. Mechanics and imaging of single DNA molecules.

7. DNA probes on beads arrayed in a capillary, 'Bead-array', exhibited high hybridization performance.

8. Differential adhesion of microspheres mediated by DNA hybridization I: experiment.

9. DNA conformation on surfaces measured by fluorescence self-interference.

10. DNA-modified electrodes fabricated using copper-free click chemistry for enhanced protein detection.