Literature DB >> 8748313

Identification of a 25-kilodalton protein of Mycobacterium bovis BCG to distinguish BCG strains from Mycobacterium tuberculosis.

D Kumar1, B S Srivastava, N B Singh, R Srivastava.   

Abstract

Mycobacterium bovis BCG vaccine strains were compared with Mycobacterium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 25-kDa protein observed in the BCG strains was absent in M. tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniquely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein and polyclonal antibodies directed against this antigen can be exploited to distinguish BCG strains from M. tuberculosis.

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Year:  1996        PMID： 8748313      PMCID： PMC228770          DOI： 10.1128/jcm.34.1.224-226.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Further studies of mycobacteriophage 33D (Warsaw) for differentiation of BCG from M. bovis and M. tubeculosis.

Authors:  W D Jones
Journal:  Tubercle       Date:  1979-03

2.  Disseminated bacille Calmette-Guérin infection in an AIDS patient 30 years after BCG vaccination.

Authors:  C Armbruster; W Junker; N Vetter; G Jaksch
Journal:  J Infect Dis       Date:  1990-11       Impact factor: 5.226

3.  Chromosomal DNA fingerprint patterns produced with IS6110 as strain-specific markers for epidemiologic study of tuberculosis.

Authors:  G H Mazurek; M D Cave; K D Eisenach; R J Wallace; J H Bates; J T Crawford
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

4.  Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids.

Authors:  M M Floyd; V A Silcox; W D Jones; W R Butler; J O Kilburn
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.

Authors:  M Harboe; S Nagai; M E Patarroyo; M L Torres; C Ramirez; N Cruz
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

8.  Intravesical therapy comparing BCG, adriamycin, and thiotepa in 200 patients with superficial bladder cancer: a randomized prospective study.

Authors:  J A Martinez-Pineiro; J Jimenez León; L Martinez-Pineiro; L Fiter; J A Mosteiro; J Navarro; M J García Matres; P Cárcamo
Journal:  Prog Clin Biol Res       Date:  1989

Review 9.  The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis.

Authors:  H G Wiker; M Harboe
Journal:  Microbiol Rev       Date:  1992-12

10.  Differentiation of strains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M. bovis BCG.

Authors:  R Frothingham
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948
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  1 in total

1.  PCR identification of Mycobacterium bovis BCG.

Authors:  E A Talbot; D L Williams; R Frothingham
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948
  1 in total

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