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Literature DB >> 8692956

Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage.

R Heller¹, K E Brown, C Burgtorf, W R Brown.

Abstract

We have used telomeric DNA to break two acrocentric derivatives of the human Y chromosome into mini-chromosomes that are small enough to be size- fractionated by pulsed-field gel electrophoresis. One of the mini-chromosomes is about 7 Mb in size and sequence-tagged site analysis of this molecule suggests that it corresponds to a simple truncation of the short arm of the Y chromosome. Five of the mini-chromosomes are derived from the long arm, are all rearranged by more than a simple truncation, and range in size from 4.0 Mb to 9 Mb. We have studied the mitotic stabilities of these mini-chromosomes and shown that they are stably maintained by cells proliferating in culture for about 100 cell divisions.

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Year: 1996 PMID： 8692956 PMCID： PMC38947 DOI： 10.1073/pnas.93.14.7125

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

10 in total

1. Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

Authors: S D Lupton; L L Brunton; V A Kalberg; R W Overell
Journal: Mol Cell Biol Date: 1991-06 Impact factor: 4.272

2. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

Authors: J B Fan; Y Chikashige; C L Smith; O Niwa; M Yanagida; C R Cantor
Journal: Nucleic Acids Res Date: 1989-04-11 Impact factor: 16.971

3. A model for the separation of large DNA molecules by crossed field gel electrophoresis.

Authors: E M Southern; R Anand; W R Brown; D S Fletcher
Journal: Nucleic Acids Res Date: 1987-08-11 Impact factor: 16.971

4. Dissecting the centromere of the human Y chromosome with cloned telomeric DNA.

Authors: K E Brown; M A Barnett; C Burgtorf; P Shaw; V J Buckle; W R Brown
Journal: Hum Mol Genet Date: 1994-08 Impact factor: 6.150

5. The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors.

Authors: A Stotz; P Linder
Journal: Gene Date: 1990-10-30 Impact factor: 3.688

6. Functional reintroduction of human telomeres into mammalian cells.

Authors: C Farr; J Fantes; P Goodfellow; H Cooke
Journal: Proc Natl Acad Sci U S A Date: 1991-08-15 Impact factor: 11.205

7. The human Y chromosome: a 43-interval map based on naturally occurring deletions.

Authors: D Vollrath; S Foote; A Hilton; L G Brown; P Beer-Romero; J S Bogan; D C Page
Journal: Science Date: 1992-10-02 Impact factor: 47.728

8. Telomere directed fragmentation of mammalian chromosomes.

Authors: M A Barnett; V J Buckle; E P Evans; A C Porter; D Rout; A G Smith; W R Brown
Journal: Nucleic Acids Res Date: 1993-01-11 Impact factor: 16.971

9. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

Authors: R C Mulligan; P Berg
Journal: Proc Natl Acad Sci U S A Date: 1981-04 Impact factor: 11.205

Review 10. Mammalian artificial chromosomes.

Authors: W R Brown
Journal: Curr Opin Genet Dev Date: 1992-06 Impact factor: 5.578

10 in total

37 in total

1. 1st International Conference on the Mammalian Centromere. Taichung, Taiwan, 2-4 October 1998. Abstracts.

Authors:
Journal: Chromosome Res Date: 1998-12 Impact factor: 5.239

2. Hypothesis: for the worst and for the best, L1Hs retrotransposons actively participate in the evolution of the human centromeric alphoid sequences.

Authors: A M Laurent; J Puechberty; G Roizès
Journal: Chromosome Res Date: 1999 Impact factor: 5.239

Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage.

1. Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

2. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

3. A model for the separation of large DNA molecules by crossed field gel electrophoresis.

4. Dissecting the centromere of the human Y chromosome with cloned telomeric DNA.

5. The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors.

6. Functional reintroduction of human telomeres into mammalian cells.

7. The human Y chromosome: a 43-interval map based on naturally occurring deletions.

8. Telomere directed fragmentation of mammalian chromosomes.

9. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

Review 10. Mammalian artificial chromosomes.

1. 1st International Conference on the Mammalian Centromere. Taichung, Taiwan, 2-4 October 1998. Abstracts.

2. Hypothesis: for the worst and for the best, L1Hs retrotransposons actively participate in the evolution of the human centromeric alphoid sequences.

3. Neocentromeres and human artificial chromosomes: an unnatural act.

Review 4. Microcell-mediated chromosome transfer (MMCT): small cells with huge potential.

5. Telomere-mediated chromosomal truncation in maize.

Review 6. Human artificial chromosomes for gene delivery and the development of animal models.

7. Construction and behavior of engineered minichromosomes in maize.

8. Meiotic transmission rates correlate with physical features of rearranged centromeres in maize.

Review 9. Chromosome manipulation: a systematic approach toward understanding human chromosome structure and function.

Review 10. A new generation of human artificial chromosomes for functional genomics and gene therapy.