Literature DB >> 1645450

Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

S D Lupton1, L L Brunton, V A Kalberg, R W Overell.   

Abstract

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

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Year:  1991        PMID： 1645450      PMCID： PMC360192          DOI： 10.1128/mcb.11.6.3374-3378.1991

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  26 in total

1.  Inhibition by ganciclovir of cell growth and DNA synthesis of cells biochemically transformed with herpesvirus genetic information.

Authors:  M H St Clair; C U Lambe; P A Furman
Journal:  Antimicrob Agents Chemother       Date:  1987-06       Impact factor: 5.191

2.  Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region.

Authors:  M A Bender; T D Palmer; R E Gelinas; A D Miller
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

Review 3.  An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971

4.  Selection against expression of the Escherichia coli gene gpt in hprt+ mouse teratocarcinoma and hybrid cells.

Authors:  C Besnard; E Monthioux; J Jami
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

5.  Stably transmitted triple-promoter retroviral vectors and their use in transformation of primary mammalian cells.

Authors:  R W Overell; K E Weisser; D Cosman
Journal:  Mol Cell Biol       Date:  1988-04       Impact factor: 4.272

6.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  Thymidine kinase obliteration: creation of transgenic mice with controlled immune deficiency.

Authors:  R A Heyman; E Borrelli; J Lesley; D Anderson; D D Richman; S M Baird; R Hyman; R M Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

8.  Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells.

Authors:  R F Santerre; N E Allen; J N Hobbs; R N Rao; R J Schmidt
Journal:  Gene       Date:  1984-10       Impact factor: 3.688

9.  Utilization of internal AUG codons for initiation of protein synthesis directed by mRNAs from normal and mutant genes encoding herpes simplex virus-specified thymidine kinase.

Authors:  L Haarr; H S Marsden; C M Preston; J R Smiley; W C Summers; W P Summers
Journal:  J Virol       Date:  1985-11       Impact factor: 5.103

10.  Lymphoma regression induced by ganciclovir in mice bearing a herpes thymidine kinase transgene.

Authors:  F L Moolten; J M Wells; R A Heyman; R M Evans
Journal:  Hum Gene Ther       Date:  1990       Impact factor: 5.695
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  50 in total

1.  Nonmyeloablative immunosuppressive regimen prolongs In vivo persistence of gene-modified autologous T cells in a nonhuman primate model.

Authors:  C Berger; M L Huang; M Gough; P D Greenberg; S R Riddell; H P Kiem
Journal:  J Virol       Date:  2001-01       Impact factor: 5.103

2.  Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position.

Authors:  Susan K Eszterhas; Eric E Bouhassira; David I K Martin; Steven Fiering
Journal:  Mol Cell Biol       Date:  2002-01       Impact factor: 4.272

3.  Construction of strains for rapid homokaryon purification after integration of constructs at the histidine-3 ( his-3) locus of Neurospora crassa.

Authors:  Dong W Lee; Jeremy R Haag; Rodolfo Aramayo
Journal:  Curr Genet       Date:  2003-02-18       Impact factor: 3.886

4.  Manipulation of T cell costimulatory and inhibitory signals for immunotherapy of prostate cancer.

Authors:  E D Kwon; A A Hurwitz; B A Foster; C Madias; A L Feldhaus; N M Greenberg; M B Burg; J P Allison
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

5.  Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite array.

Authors:  Jennifer M Spence; Ricky Critcher; Thomas A Ebersole; Manuel M Valdivia; William C Earnshaw; Tatsuo Fukagawa; Christine J Farr
Journal:  EMBO J       Date:  2002-10-01       Impact factor: 11.598

6.  An argonaute-like protein is required for meiotic silencing.

Authors:  Dong W Lee; Robert J Pratt; Malcolm McLaughlin; Rodolfo Aramayo
Journal:  Genetics       Date:  2003-06       Impact factor: 4.562

7.  Unpaired genes do not silence their paired neighbors.

Authors:  Brandi L Kutil; Kye-Yong Seong; Rodolfo Aramayo
Journal:  Curr Genet       Date:  2003-06-12       Impact factor: 3.886

8.  Shuttle vectors conferring hygromycin B resistance to E. coli and to mammalian cells. Differential expression of carboxyterminal fusion proteins.

Authors:  F A Asselbergs; R Pronk
Journal:  Mol Biol Rep       Date:  1992-11       Impact factor: 2.316

9.  Differentiation of naive cord-blood T cells into CD19-specific cytolytic effectors for posttransplantation adoptive immunotherapy.

Authors:  Lisa Marie Serrano; Timothy Pfeiffer; Simon Olivares; Tontanai Numbenjapon; Jennifer Bennitt; Daniel Kim; David Smith; George McNamara; Zaid Al-Kadhimi; Joseph Rosenthal; Stephen J Forman; Michael C Jensen; Laurence J N Cooper
Journal:  Blood       Date:  2005-12-13       Impact factor: 22.113

10.  A dominant positive and negative selectable gene for use in mammalian cells.

Authors:  F Schwartz; N Maeda; O Smithies; R Hickey; W Edelmann; A Skoultchi; R Kucherlapati
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205
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