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Literature DB >> 866182

Lac repressor purification without inactivation of DNA binding activity.

J M Rosenberg, O B Khallai, M L Kopka, R E Dickerson, A D Riggs.

Abstract

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 +/- 10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.

Mesh：

Substances：
DNA, Viral
Lactose

Year: 1977 PMID： 866182 PMCID： PMC342462 DOI： 10.1093/nar/4.3.567

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

10 in total

1. Binding of lactose repressor to poly d(A-T) : OD AND CD melting of the complex.

Authors: R Clement; M P Daune
Journal: Nucleic Acids Res Date: 1975-03 Impact factor: 16.971

2. Interaction of effecting ligands with lac repressor and repressor-operator complex.

Authors: M D Barkley; A D Riggs; A Jobe; S Burgeois
Journal: Biochemistry Date: 1975-04-22 Impact factor: 3.162

3. Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity.

Authors: T Platt; J G Files; K Weber
Journal: J Biol Chem Date: 1973-01-10 Impact factor: 5.157

4. Lac repressor binding to non-operator DNA: detailed studies and a comparison of eequilibrium and rate competition methods.

Authors: S Y Lin; A D Riggs
Journal: J Mol Biol Date: 1972-12-30 Impact factor: 5.469

5. Equilibrium and kinetic studies of Escherichia coli lac repressor-inducer interactions.

Authors: S L Laiken; C A Gross; P H Von Hippel
Journal: J Mol Biol Date: 1972-04-28 Impact factor: 5.469

6. Characterization of the lac repressor species produced by limited tryptic cleavage.

Authors: J S Huston; W F Foo-Penn; K C Bechtel; O Jardetzky
Journal: Biochem Biophys Res Commun Date: 1974-11-27 Impact factor: 3.575

7. lac repressor--operator interaction. II. Effect of galactosides and other ligands.

Authors: A D Riggs; R F Newby; S Bourgeois
Journal: J Mol Biol Date: 1970-07-28 Impact factor: 5.469

8. On the assay, isolation and characterization of the lac repressor.

Authors: A D Riggs; S Bourgeois
Journal: J Mol Biol Date: 1968-07-14 Impact factor: 5.469

9. Lac repressor-operator interaction. I. Equilibrium studies.

Authors: A D Riggs; H Suzuki; S Bourgeois
Journal: J Mol Biol Date: 1970-02-28 Impact factor: 5.469

10. Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.

Authors: S Y Lin; A D Riggs
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

10 in total

12 in total

1. Use of urea and glycine betaine to quantify coupled folding and probe the burial of DNA phosphates in lac repressor-lac operator binding.

Authors: Jiang Hong; Mike W Capp; Ruth M Saecker; M Thomas Record
Journal: Biochemistry Date: 2005-12-27 Impact factor: 3.162

2. Lac repressor-Lac operator complexes. Solution X-ray scattering and electrophoretic studies.

Authors: F Culard; M Charlier; J C Maurizot; A Tardieu
Journal: Eur Biophys J Date: 1987 Impact factor: 1.733

3. Methylphosphonates as probes of protein-nucleic acid interactions.

Authors: S A Noble; E F Fisher; M H Caruthers
Journal: Nucleic Acids Res Date: 1984-04-11 Impact factor: 16.971

4. Lac repressor - lac operator interaction. Circular dichroism study.

Authors: F Culard; J C Maurizot
Journal: Nucleic Acids Res Date: 1981-10-10 Impact factor: 16.971

5. Perturbation of lac operator DNA modification by tryptic core protein from lac repressor.

Authors: S P Manly; G N Bennett; K S Matthews
Journal: Proc Natl Acad Sci U S A Date: 1983-10 Impact factor: 11.205

6. A perfectly symmetric lac operator binds the lac repressor very tightly.

Authors: J R Sadler; H Sasmor; J L Betz
Journal: Proc Natl Acad Sci U S A Date: 1983-11 Impact factor: 11.205

7. Site-specific DNA-affinity chromatography of the lac repressor.

Authors: G Herrick
Journal: Nucleic Acids Res Date: 1980-08-25 Impact factor: 16.971

8. Protein-induced unwinding of DNA: measurement by gel electrophoresis of complexes with DNA minicircles. Application to restriction endonuclease EcoRI, catabolite gene activator protein and lac repressor.

Authors: S Douc-Rasy; A Kolb; A Prunell
Journal: Nucleic Acids Res Date: 1989-07-11 Impact factor: 16.971

9. 5-Azido-2'-deoxyuridine 5'-triphosphate: a photoaffinity-labeling reagent and tool for the enzymatic synthesis of photoactive DNA.

Authors: R K Evans; J D Johnson; B E Haley
Journal: Proc Natl Acad Sci U S A Date: 1986-08 Impact factor: 11.205

10. Interaction between the lac operator and the lac repressor headpiece: fluorescence and circular dichroism studies.

Authors: F Culard; M Schnarr; J C Maurizot
Journal: EMBO J Date: 1982 Impact factor: 11.598