Literature DB >> 8657583

Improved cloning of antibody variable regions from hybridomas by an antisense-directed RNase H digestion of the P3-X63-Ag8.653 derived pseudogene mRNA.

C Ostermeier1, H Michel.   

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Year:  1996        PMID： 8657583      PMCID： PMC145887          DOI： 10.1093/nar/24.10.1979

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  8 in total

1.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

2.  Complete variable region sequence of a nonfunctionally rearranged kappa light chain transcribed in the nonsecretor P3-X63-Ag8.653 myeloma cell line.

Authors:  R Strohal; G Kroemer; G Wick; R Kofler
Journal:  Nucleic Acids Res       Date:  1987-03-25       Impact factor: 16.971

3.  Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.

Authors:  R Orlandi; D H Güssow; P T Jones; G Winter
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

4.  Hybridoma fusion cell lines contain an aberrant kappa transcript.

Authors:  W L Carroll; E Mendel; S Levy
Journal:  Mol Immunol       Date:  1988-10       Impact factor: 4.407

5.  An improved method for generating single-chain antibodies from hybridomas.

Authors:  P J Nicholls; V G Johnson; M D Blanford; S M Andrew
Journal:  J Immunol Methods       Date:  1993-09-27       Impact factor: 2.303

6.  Molecular characterization of a nonsecreting myeloma mutant.

Authors:  Y Argon; O R Burrone; C Milstein
Journal:  Eur J Immunol       Date:  1983-04       Impact factor: 5.532

7.  Site specific enzymatic cleavage of RNA.

Authors:  H Donis-Keller
Journal:  Nucleic Acids Res       Date:  1979-09-11       Impact factor: 16.971

8.  Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes.

Authors:  G Kleymann; C Ostermeier; B Ludwig; A Skerra; H Michel
Journal:  Biotechnology (N Y)       Date:  1995-02
  8 in total
  6 in total

1.  Identification of two aberrant transcripts derived from a hybridoma with amplification of functional immunoglobulin variable genes.

Authors:  Guipeng Ding; Ximin Chen; Jin Zhu; Brian Cao
Journal:  Cell Mol Immunol       Date:  2010-07-26       Impact factor: 11.530

2.  Molecular engineering of high affinity single-chain antibody fragment for endothelial targeting of proteins and nanocarriers in rodents and humans.

Authors:  Colin F Greineder; Elizabeth D Hood; Anning Yao; Makan Khoshnejad; Jake S Brenner; Ian H Johnston; Mortimer Poncz; Claudia Gottstein; Vladimir R Muzykantov
Journal:  J Control Release       Date:  2016-02-06       Impact factor: 9.776

Review 3.  Recombinant antibodies and their use for food immunoanalysis.

Authors:  Riikka Peltomaa; Rodrigo Barderas; Elena Benito-Peña; María C Moreno-Bondi
Journal:  Anal Bioanal Chem       Date:  2021-08-21       Impact factor: 4.142

4.  A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.

Authors:  Ghasidit Pornnoppadol; Boya Zhang; Alec A Desai; Anthony Berardi; Henriette A Remmer; Peter M Tessier; Colin F Greineder
Journal:  PLoS One       Date:  2021-10-11       Impact factor: 3.752

5.  Cloning and plant-based production of antibody MC10E7 for a lateral flow immunoassay to detect [4-arginine]microcystin in freshwater.

Authors:  Stanislav Melnik; Anna-Cathrine Neumann; Ryan Karongo; Sebastian Dirndorfer; Martin Stübler; Verena Ibl; Reinhard Niessner; Dietmar Knopp; Eva Stoger
Journal:  Plant Biotechnol J       Date:  2017-06-05       Impact factor: 13.263

6.  When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions.

Authors:  Andrew R M Bradbury; Nathan D Trinklein; Holger Thie; Ian C Wilkinson; Atul K Tandon; Stephen Anderson; Catherine L Bladen; Brittany Jones; Shelley Force Aldred; Marco Bestagno; Oscar Burrone; Jennifer Maynard; Fortunato Ferrara; James S Trimmer; Janina Görnemann; Jacob Glanville; Philipp Wolf; Andre Frenzel; Julin Wong; Xin Yu Koh; Hui-Yan Eng; David Lane; Marie-Paule Lefranc; Mike Clark; Stefan Dübel
Journal:  MAbs       Date:  2018-03-29       Impact factor: 5.857
  6 in total

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