Literature DB >> 3146025

Hybridoma fusion cell lines contain an aberrant kappa transcript.

W L Carroll1, E Mendel, S Levy.   

Abstract

The V region sequence of a non-productive kappa transcript from two myeloma fusion partners has been determined. This transcript has an aberrant VJ recombination site resulting in a translation stop site at position 105. It is variably expressed in hybridomas made from all fusion partners derived from the original MOPC-21 tumor. The amount of this transcript may greatly exceed levels of the productive light chain mRNA.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3146025     DOI： 10.1016/0161-5890(88)90005-3

Source DB:  PubMed          Journal:  Mol Immunol        ISSN： 0161-5890            Impact factor:   4.407


  25 in total

1.  Hybridoma passage in vitro may result in reduced ability of antimannan antibody to protect against disseminated candidiasis.

Authors:  Hong Xin; Jim E Cutler
Journal:  Infect Immun       Date:  2006-07       Impact factor: 3.441

2.  Impact of the molecular form of immunoglobulin A on functional activity in defense against Streptococcus pneumoniae.

Authors:  Claudine E Fasching; Tracy Grossman; Blaise Corthésy; Andrew G Plaut; Jeffrey N Weiser; Edward N Janoff
Journal:  Infect Immun       Date:  2007-01-29       Impact factor: 3.441

3.  Allelic variants of rearranged immunoglobulin heavy and light chain genes in hybridoma PTF-02 and parent myeloma.

Authors:  S M Deyev; D N Urakov; A G Stepchenko; O L Polanovsky
Journal:  Genetica       Date:  1991       Impact factor: 1.082

4.  Improved cloning of antibody variable regions from hybridomas by an antisense-directed RNase H digestion of the P3-X63-Ag8.653 derived pseudogene mRNA.

Authors:  C Ostermeier; H Michel
Journal:  Nucleic Acids Res       Date:  1996-05-15       Impact factor: 16.971

5.  Inherent anti-amyloidogenic activity of human immunoglobulin gamma heavy chains.

Authors:  Sharad P Adekar; Igor Klyubin; Sally Macy; Michael J Rowan; Alan Solomon; Scott K Dessain; Brian O'Nuallain
Journal:  J Biol Chem       Date:  2009-11-04       Impact factor: 5.157

6.  A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration.

Authors:  Jean-Christophe Peter; Anne-Catherine Lecourt; Marjorie Weckering; Géraldine Zipfel; Michael L Niehoff; William A Banks; Karl G Hofbauer
Journal:  J Pharmacol Exp Ther       Date:  2010-01-29       Impact factor: 4.030

7.  Identification of two aberrant transcripts derived from a hybridoma with amplification of functional immunoglobulin variable genes.

Authors:  Guipeng Ding; Ximin Chen; Jin Zhu; Brian Cao
Journal:  Cell Mol Immunol       Date:  2010-07-26       Impact factor: 11.530

8.  Epstein-Barr viruses that express a CD21 antibody provide evidence that gp350's functions extend beyond B-cell surface binding.

Authors:  Clemens Busse; Regina Feederle; Martina Schnölzer; Uta Behrends; Josef Mautner; Henri-Jacques Delecluse
Journal:  J Virol       Date:  2009-11-04       Impact factor: 5.103

9.  Construction and expression of a bifunctional single-chain antibody against Bacillus cereus p6ores.

Authors:  K Koo; P M Foegeding; H E Swaisgood
Journal:  Appl Environ Microbiol       Date:  1998-07       Impact factor: 4.792

10.  Elimination of endogenous aberrant kappa chain transcripts from sp2/0-derived hybridoma cells by specific ribozyme cleavage: utility in genetic therapy of HIV-1 infections.

Authors:  L Duan; R J Pomerantz
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.