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Literature DB >> 8648756

Cloning, expression and characterization of the proteinase from human herpesvirus 6.

N J Tigue¹, P J Matharu, N A Roberts, J S Mills, J Kay, R Jupp.

Abstract

After the U53 gene encoding the proteinase from human herpesvirus 6 (HHV-6) was sequenced, it was expressed in Escherichia coli, and the activity of the purified, recombinant HHV-6 proteinase was characterized quantitatively by using synthetic peptide substrates mimicking the release and maturation cleavage sites in the polyprotein precursors of HHV-6, human cytomegalovirus (CMV), murine CMV, and Epstein-Barr virus. Despite sharing 40% identity with other betaherpesvirus proteinases such as human CMV proteinase, the one-chain HHV-6 enzyme was distinguished from these two-chain proteinases by the absence of an internal autocatalytic cleavage site.

Entities: Gene Species

Mesh：

Substances：

Year: 1996 PMID： 8648756 PMCID： PMC190303

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

30 in total

1. The protease of herpes simplex virus type 1 is essential for functional capsid formation and viral growth.

Authors: M Gao; L Matusick-Kumar; W Hurlburt; S F DiTusa; W W Newcomb; J C Brown; P J McCann; I Deckman; R J Colonno
Journal: J Virol Date: 1994-06 Impact factor: 5.103

2. Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase.

Authors: G Donaghy; R Jupp
Journal: J Virol Date: 1995-02 Impact factor: 5.103

3. The herpes simplex virus gene UL26 proteinase in the presence of the UL26.5 gene product promotes the formation of scaffold-like structures.

Authors: V G Preston; M F al-Kobaisi; I M McDougall; F J Rixon
Journal: J Gen Virol Date: 1994-09 Impact factor: 3.891

4. Cloning and sequence analysis of murine cytomegalovirus protease and capsid assembly protein genes.

Authors: J M Loutsch; N J Galvin; M L Bryant; B C Holwerda
Journal: Biochem Biophys Res Commun Date: 1994-08-30 Impact factor: 3.575

Review 5. Alphavirus positive and negative strand RNA synthesis and the role of polyproteins in formation of viral replication complexes.

Authors: D L Sawicki; S G Sawicki
Journal: Arch Virol Suppl Date: 1994

Cloning, expression and characterization of the proteinase from human herpesvirus 6.

1. The protease of herpes simplex virus type 1 is essential for functional capsid formation and viral growth.

2. Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase.

3. The herpes simplex virus gene UL26 proteinase in the presence of the UL26.5 gene product promotes the formation of scaffold-like structures.

4. Cloning and sequence analysis of murine cytomegalovirus protease and capsid assembly protein genes.

Review 5. Alphavirus positive and negative strand RNA synthesis and the role of polyproteins in formation of viral replication complexes.

6. The effect of internal autocleavage on kinetic properties of the human cytomegalovirus protease catalytic domain.

7. Activity of two-chain recombinant human cytomegalovirus protease.

8. Cytomegalovirus protein substrates are not cleaved by the herpes simplex virus type 1 proteinase.

9. In vitro proteolytic activity and active-site identification of the human cytomegalovirus protease.

Review 10. Ganciclovir. An update of its therapeutic use in cytomegalovirus infection.

1. Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus.

2. Comparison of the complete DNA sequences of human herpesvirus 6 variants A and B.

3. Expression and characterization of recombinant murine cytomegalovirus protease.

4. The protease and the assembly protein of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8).

Review 5. Human herpesvirus 6: An emerging pathogen.

Review 6. Molecular and biotechnological aspects of microbial proteases.