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Literature DB >> 7815503

Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase.

Abstract

The BVRF2 gene of Epstein-Barr virus (EBV) shows homology to the UL26 and UL80 genes of herpes simplex virus type 1 (HSV-1) and cytomegalovirus (CMV), respectively. These genes are believed to provide a scaffold protein for the assembly of capsids leading to the formation of infectious viral particles. We have cloned the BVRF2 gene from the B95.8 strain of EBV and shown that the BVRF2 gene product is a polyprotein capable of autoproteolytic cleavage. Two Ala-Ser-containing recognition sequences were identified in the BVRF2 polyprotein at amino acid positions 568/569 and 570/571 where this cleavage was expected to occur. Here, we show that EBV proteinase is capable of cleaving at the first Ala-Ser bond but not the second. Comparison of the processing of the EBV and human CMV assembly domains in vitro by either EBV or human CMV proteinase revealed that, while both proteinases could cleave their native assembly domain, only EBV proteinase was able to cleave the assembly domain of the other virus.

Entities: Chemical Gene Species

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Year: 1995 PMID： 7815503 PMCID： PMC188701

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

25 in total

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Authors: C J Ring
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5. Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA.

Authors: V G Preston; J A Coates; F J Rixon
Journal: J Virol Date: 1983-03 Impact factor: 5.103

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9. Human cytomegalovirus IE86 protein interacts with promoter-bound TATA-binding protein via a specific region distinct from the autorepression domain.

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12 in total

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2. Substrate modulation of enzyme activity in the herpesvirus protease family.

Authors: Ana Lazic; David H Goetz; Anson M Nomura; Alan B Marnett; Charles S Craik
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3. Assembly of Epstein-Barr Virus Capsid in Promyelocytic Leukemia Nuclear Bodies.

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4. Antibodies to lytic infection proteins in lymphocryptovirus-infected rhesus macaques: a model for humoral immune responses to epstein-barr virus infection.

Authors: Nina Orlova; Mark H Fogg; Angela Carville; Fred Wang
Journal: Clin Vaccine Immunol Date: 2011-07-06

5. Sequential autoprocessing of Marek's disease herpesvirus protease differs from that of other herpesviruses.

Authors: S Laurent; C Blondeau; M Belghazi; S Remy; E Esnault; P Rasschaert; D Rasschaert
Journal: J Virol Date: 2007-03-21 Impact factor: 5.103

6. Virus-specific interaction between the human cytomegalovirus major capsid protein and the C terminus of the assembly protein precursor.

Authors: M Beaudet-Miller; R Zhang; J Durkin; W Gibson; A D Kwong; Z Hong
Journal: J Virol Date: 1996-11 Impact factor: 5.103

7. Identification of a minimal hydrophobic domain in the herpes simplex virus type 1 scaffolding protein which is required for interaction with the major capsid protein.

Authors: Z Hong; M Beaudet-Miller; J Durkin; R Zhang; A D Kwong
Journal: J Virol Date: 1996-01 Impact factor: 5.103