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Literature DB >> 9261446

Expression and characterization of recombinant murine cytomegalovirus protease.

J H Sloan¹, J M Loutsch, S Y Boyce, B C Holwerda.

Abstract

The protease domain of the murine cytomegalovirus (MCMV) M80 open reading frame was expressed in and purified from Escherichia coli. The recombinant enzyme was recovered as a mixture of active one- and two-chain forms. The two-chain enzyme was formed by internal cleavage of the one-chain enzyme at the I site. Activity measurements showed that MCMV protease cleaves R- and M-site peptide mimics with kinetics similar to those of recombinant human cytomegalovirus (HCMV) protease. Both the MCMV and HCMV proteases cleave I-site peptide substrates very poorly, but the crystal structure of the HCMV protease indicates that the cytomegalovirus I site likely resides on a solvent-exposed loop close to the active site.

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Year: 1997 PMID： 9261446 PMCID： PMC192009

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

25 in total

1. Structure of the human cytomegalovirus protease catalytic domain reveals a novel serine protease fold and catalytic triad.

Authors: P Chen; H Tsuge; R J Almassy; C L Gribskov; S Katoh; D L Vanderpool; S A Margosiak; C Pinko; D A Matthews; C C Kan
Journal: Cell Date: 1996-09-06 Impact factor: 41.582

2. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells.

Authors: M R Hall; W Gibson
Journal: J Virol Date: 1996-08 Impact factor: 5.103

3. Proteolytic activity of human cytomegalovirus UL80 protease cleavage site mutants.

Authors: T R Jones; L Sun; G A Bebernitz; V P Muzithras; H J Kim; S H Johnston; E Z Baum
Journal: J Virol Date: 1994-06 Impact factor: 5.103

4. Cloning and sequence analysis of murine cytomegalovirus protease and capsid assembly protein genes.

Authors: J M Loutsch; N J Galvin; M L Bryant; B C Holwerda
Journal: Biochem Biophys Res Commun Date: 1994-08-30 Impact factor: 3.575

5. Analysis of the complete DNA sequence of murine cytomegalovirus.

Authors: W D Rawlinson; H E Farrell; B G Barrell
Journal: J Virol Date: 1996-12 Impact factor: 5.103

6. A new serine-protease fold revealed by the crystal structure of human cytomegalovirus protease.

Authors: L Tong; C Qian; M J Massariol; P R Bonneau; M G Cordingley; L Lagacé
Journal: Nature Date: 1996-09-19 Impact factor: 49.962

7. The effect of internal autocleavage on kinetic properties of the human cytomegalovirus protease catalytic domain.

Authors: D R O'Boyle; K Wager-Smith; J T Stevens; S P Weinheimer
Journal: J Biol Chem Date: 1995-03-03 Impact factor: 5.157

8. Cloning, expression and characterization of the proteinase from human herpesvirus 6.

Authors: N J Tigue; P J Matharu; N A Roberts; J S Mills; J Kay; R Jupp
Journal: J Virol Date: 1996-06 Impact factor: 5.103

9. Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

Authors: P J Burck; D H Berg; T P Luk; L M Sassmannshausen; M Wakulchik; D P Smith; H M Hsiung; G W Becker; W Gibson; E C Villarreal
Journal: J Virol Date: 1994-05 Impact factor: 5.103

Expression and characterization of recombinant murine cytomegalovirus protease.

1. Structure of the human cytomegalovirus protease catalytic domain reveals a novel serine protease fold and catalytic triad.

2. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells.

3. Proteolytic activity of human cytomegalovirus UL80 protease cleavage site mutants.

4. Cloning and sequence analysis of murine cytomegalovirus protease and capsid assembly protein genes.

5. Analysis of the complete DNA sequence of murine cytomegalovirus.

6. A new serine-protease fold revealed by the crystal structure of human cytomegalovirus protease.

7. The effect of internal autocleavage on kinetic properties of the human cytomegalovirus protease catalytic domain.

8. Cloning, expression and characterization of the proteinase from human herpesvirus 6.

9. Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

10. Activity of two-chain recombinant human cytomegalovirus protease.

1. Effective inhibition in animals of viral pathogenesis by a ribozyme derived from RNase P catalytic RNA.

2. Engineered RNase P Ribozymes Effectively Inhibit the Infection of Murine Cytomegalovirus in Animals.

3. Retinal and Choroidal Pathologies in Aged BALB/c Mice Following Systemic Neonatal Murine Cytomegalovirus Infection.

4. Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences.