Literature DB >> 8627727

Purification and characterization of herpes simplex virus type 1 alkaline exonuclease expressed in Escherichia coli.

J C Bronstein1, P C Weber.   

Abstract

The alkaline exonuclease (AE) encoded by the herpes simplex virus type 1 (HSV-1) UL12 open reading frame was inducibly expressed in Escherichia coli and purified without the use of chromatographic separation. This recombinant AE was found to exhibit the same biochemical properties as the virus-encoded protein and was used to confirm the existence of a weak endonucleolytic activity in the enzyme. Antisera raised against the recombinant protein recognized several forms of the AE in HSV-1-infected cells. This expression and purification strategy will provide an economical and easily accessible alternative source of HSV-1 AE for future in vitro studies.

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Year:  1996        PMID： 8627727      PMCID： PMC190030     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  12 in total

1.  The deoxyribonuclease induced after infection of KB cells by herpes simplex virus type 1 or type 2. I. Purification and characterization of the enzyme.

Authors:  P J Hoffmann; Y C Cheng
Journal:  J Biol Chem       Date:  1978-05-25       Impact factor: 5.157

2.  The characterization of the EBV alkaline deoxyribonuclease cloned and expressed in E. coli.

Authors:  S A Baylis; D J Purifoy; E Littler
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971

3.  DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes.

Authors:  D J McGeoch; A Dolan; M C Frame
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

4.  Herpes simplex virus non-structural proteins. IV. Purification of the virus-induced deoxyribonuclease and characterization of the enzyme using monoclonal antibodies.

Authors:  L Banks; D J Purifoy; P F Hurst; R A Killington; K L Powell
Journal:  J Gen Virol       Date:  1983-10       Impact factor: 3.891

5.  High-resolution characterization of herpes simplex virus type 1 transcripts encoding alkaline exonuclease and a 50,000-dalton protein tentatively identified as a capsid protein.

Authors:  R H Costa; K G Draper; L Banks; K L Powell; G Cohen; R Eisenberg; E K Wagner
Journal:  J Virol       Date:  1983-12       Impact factor: 5.103

6.  Alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzyme.

Authors:  M Strobel-Fidler; B Francke
Journal:  Virology       Date:  1980-06       Impact factor: 3.616

7.  Characterization of the genes encoding herpes simplex virus type 1 and type 2 alkaline exonucleases and overlapping proteins.

Authors:  K G Draper; G Devi-Rao; R H Costa; E D Blair; R L Thompson; E K Wagner
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

8.  The herpes simplex virus type 1 alkaline nuclease is not essential for viral DNA synthesis: isolation and characterization of a lacZ insertion mutant.

Authors:  S K Weller; M R Seghatoleslami; L Shao; D Rowse; E P Carmichael
Journal:  J Gen Virol       Date:  1990-12       Impact factor: 3.891

9.  Purification and properties of Epstein-Barr virus DNase expressed in Escherichia coli.

Authors:  M C Stolzenberg; T Ooka
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

10.  DNase induced after infection of KB cells by herpes simplex virus type 1 or type 2. II. Characterization of an associated endonuclease activity.

Authors:  P J Hoffmann; Y C Cheng
Journal:  J Virol       Date:  1979-11       Impact factor: 5.103
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  14 in total

1.  Characterization of a baculovirus alkaline nuclease.

Authors:  L Li; G F Rohrmann
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

2.  DNA mismatch repair proteins are required for efficient herpes simplex virus 1 replication.

Authors:  Kareem N Mohni; Adam S Mastrocola; Ping Bai; Sandra K Weller; Christopher D Heinen
Journal:  J Virol       Date:  2011-09-28       Impact factor: 5.103

3.  Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments.

Authors:  Armel Nicolas; Nathalie Alazard-Dany; Coline Biollay; Loredana Arata; Nelly Jolinon; Lauriane Kuhn; Myriam Ferro; Sandra K Weller; Alberto L Epstein; Anna Salvetti; Anna Greco
Journal:  J Virol       Date:  2010-06-23       Impact factor: 5.103

4.  The product of the UL12.5 gene of herpes simplex virus type 1 is a capsid-associated nuclease.

Authors:  J C Bronstein; S K Weller; P C Weber
Journal:  J Virol       Date:  1997-04       Impact factor: 5.103

5.  The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus.

Authors:  Nina Bacher Reuven; Susumu Antoku; Sandra K Weller
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

6.  Varicella-zoster virus open reading frame 48 encodes an active nuclease.

Authors:  Niklaus H Mueller; Don Gilden; Randall J Cohrs
Journal:  J Virol       Date:  2013-08-21       Impact factor: 5.103

7.  Baculovirus alkaline nuclease possesses a 5'-->3' exonuclease activity and associates with the DNA-binding protein LEF-3.

Authors:  Victor S Mikhailov; Kazuhiro Okano; George F Rohrmann
Journal:  J Virol       Date:  2003-02       Impact factor: 5.103

8.  Mitochondrial nucleases ENDOG and EXOG participate in mitochondrial DNA depletion initiated by herpes simplex virus 1 UL12.5.

Authors:  Brett A Duguay; James R Smiley
Journal:  J Virol       Date:  2013-08-28       Impact factor: 5.103

9.  In vitro processing of herpes simplex virus type 1 DNA replication intermediates by the viral alkaline nuclease, UL12.

Authors:  J N Goldstein; S K Weller
Journal:  J Virol       Date:  1998-11       Impact factor: 5.103

10.  Crystal structure of a KSHV-SOX-DNA complex: insights into the molecular mechanisms underlying DNase activity and host shutoff.

Authors:  Claire Bagnéris; Louise C Briggs; Renos Savva; Bahram Ebrahimi; Tracey E Barrett
Journal:  Nucleic Acids Res       Date:  2011-03-17       Impact factor: 16.971
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