| Literature DB >> 8625430 |
G Mernitz1, A Koch, B Henrissat, G Schulz.
Abstract
The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcription startpoints were identified. Five motifs matching the binding site of the carbon-catabolite repressor CREA of A. nidulans were detected. Their potential implication in repression was analyzed by bandshift assays.Entities:
Mesh:
Substances:
Year: 1996 PMID: 8625430 DOI: 10.1007/bf02221519
Source DB: PubMed Journal: Curr Genet ISSN: 0172-8083 Impact factor: 3.886