Literature DB >> 8093602

Identification of an essential glutamate residue in the active site of endoglucanase III from Trichoderma reesei.

R Macarron1, J van Beeumen, B Henrissat, I de la Mata, M Claeyssens.   

Abstract

n-Propyl, n-butyl and n-pentyl beta-cellobiosides with a reactive omega-epoxide in their aglycon completely and irreversibly inactivate endoglucanase III from Trichoderma reesei. The pentyl derivative was found to be most effective. From these affinity labeling experiments evidence was found for the implication of Glu329 in the reaction mechanism. This is discussed in relation to other structural/functional data known for endoglucanase III and several other family A glycanases.

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Year:  1993        PMID： 8093602     DOI： 10.1016/0014-5793(93)81202-b

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  12 in total

1.  A gene encoding a novel multidomain beta-1,4-mannanase from Caldibacillus cellulovorans and action of the recombinant enzyme on kraft pulp.

Authors:  A Sunna; M D Gibbs; C W Chin; P J Nelson; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

Review 2.  Approaches to labeling and identification of active site residues in glycosidases.

Authors:  S G Withers; R Aebersold
Journal:  Protein Sci       Date:  1995-03       Impact factor: 6.725

3.  High-temperature enzymatic breakdown of cellulose.

Authors:  Hongliang Wang; Fabio Squina; Fernando Segato; Andrew Mort; David Lee; Kirk Pappan; Rolf Prade
Journal:  Appl Environ Microbiol       Date:  2011-06-17       Impact factor: 4.792

4.  Epoxyalkyl glycosides of D-xylose and xylo-oligosaccharides are active-site markers of xylanases from glycoside hydrolase family 11, not from family 10.

Authors:  P Ntarima; W Nerinckx; K Klarskov; B Devreese; M K Bhat; J Van Beeumen; M Claeyssens
Journal:  Biochem J       Date:  2000-05-01       Impact factor: 3.857

5.  Gene cloning, DNA sequencing, and expression of thermostable beta-mannanase from Bacillus stearothermophilus.

Authors:  N Ethier; G Talbot; J Sygusch
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

6.  Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis.

Authors:  G Mernitz; A Koch; B Henrissat; G Schulz
Journal:  Curr Genet       Date:  1996-04       Impact factor: 3.886

7.  Molecular characterization and heterologous expression of an endo-beta-1,6-glucanase gene from the mycoparasitic fungus Trichoderma harzianum.

Authors:  J M Lora; J De la Cruz; A Llobell; T Benítez; J A Pintor-Toro
Journal:  Mol Gen Genet       Date:  1995-06-10

8.  Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain.

Authors:  H Stålbrand; A Saloheimo; J Vehmaanperä; B Henrissat; M Penttilä
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

9.  Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber.

Authors:  Fernando Segato; André R L Damasio; Thiago Augusto Gonçalves; Mario T Murakami; Fabio M Squina; Mariadelourdestm Polizeli; Andrew J Mort; Rolf A Prade
Journal:  Biotechnol Biofuels       Date:  2012-04-11       Impact factor: 6.040

10.  Cloning and biochemical characterization of an endo-1,4-β-mannanase from the coffee berry borer Hypothenemus hampei.

Authors:  Carolina Aguilera-Gálvez; Juan J Vásquez-Ospina; Pablo Gutiérrez-Sanchez; Ricardo Acuña-Zornosa
Journal:  BMC Res Notes       Date:  2013-08-22
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