Literature DB >> 8576057

Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.

V Pelicic1, J M Reyrat, B Gicquel.   

Abstract

Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.

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Year:  1996        PMID： 8576057      PMCID： PMC177784          DOI： 10.1128/jb.178.4.1197-1199.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

Review 1.  Advances in mycobacterial genetics: new promises for old diseases.

Authors:  W R Jacobs
Journal:  Immunobiology       Date:  1992-02       Impact factor: 3.144

2.  Structure and functions of the cell envelope in relation to mycobacterial virulence, pathogenicity and multiple drug resistance. 7th Forum in Microbiology.

Authors: 
Journal:  Res Microbiol       Date:  1991-05       Impact factor: 3.992

3.  Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans.

Authors:  W Jäger; A Schäfer; A Pühler; G Labes; W Wohlleben
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

4.  Insertional mutagenesis and illegitimate recombination in mycobacteria.

Authors:  G V Kalpana; B R Bloom; W R Jacobs
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

5.  Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon.

Authors:  I C Blomfield; V Vaughn; R F Rest; B I Eisenstein
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

6.  Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg.

Authors:  J A Lepesant; F Kunst; J Lepesant-Kejzlarová; R Dedonder
Journal:  Mol Gen Genet       Date:  1972

7.  An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis.

Authors:  J L Ried; A Collmer
Journal:  Gene       Date:  1987       Impact factor: 3.688

8.  Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.

Authors:  S B Snapper; R E Melton; S Mustafa; T Kieser; W R Jacobs
Journal:  Mol Microbiol       Date:  1990-11       Impact factor: 3.501

9.  Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a "mini" mycobacterium-Escherichia coli shuttle vector.

Authors:  M G Ranes; J Rauzier; M Lagranderie; M Gheorghiu; B Gicquel
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

10.  Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.

Authors:  P Gay; D Le Coq; M Steinmetz; T Berkelman; C I Kado
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490
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  40 in total

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Authors:  Sreedhar R Nallapareddy; Kavindra V Singh; Barbara E Murray
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

4.  Two dd-Carboxypeptidases from Mycobacterium smegmatis Affect Cell Surface Properties through Regulation of Peptidoglycan Cross-Linking and Glycopeptidolipids.

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5.  Construction and characterization of a Mycobacterium tuberculosis mutant lacking the alternate sigma factor gene, sigF.

Authors:  P Chen; R E Ruiz; Q Li; R F Silver; W R Bishai
Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

Review 6.  Counterselectable markers: untapped tools for bacterial genetics and pathogenesis.

Authors:  J M Reyrat; V Pelicic; B Gicquel; R Rappuoli
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

7.  Characterization of the mIHF gene of Mycobacterium smegmatis.

Authors:  M L Pedulla; G F Hatfull
Journal:  J Bacteriol       Date:  1998-10       Impact factor: 3.490

8.  Two nonredundant SecA homologues function in mycobacteria.

Authors:  M Braunstein; A M Brown; S Kurtz; W R Jacobs
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

9.  Characterization of the basic replicon of Rhodococcus plasmid pSOX and development of a Rhodococcus-Escherichia coli shuttle vector.

Authors:  C Denis-Larose; H Bergeron; D Labbé; C W Greer; J Hawari; M J Grossman; B M Sankey; P C Lau
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

Review 10.  Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence.

Authors:  Issar Smith
Journal:  Clin Microbiol Rev       Date:  2003-07       Impact factor: 26.132
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