Literature DB >> 2997137

Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.

P Gay, D Le Coq, M Steinmetz, T Berkelman, C I Kado.   

Abstract

We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene is inactivated. By using pUCD800, we isolated a 1.4-kilobase putative IS element from Agrobacterium tumefaciens NT1RE by selection for growth on sucrose medium. This putative IS element appears to be unique to Agrobacterium strains.

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Year:  1985        PMID： 2997137      PMCID： PMC214340          DOI： 10.1128/jb.164.2.918-921.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.

Authors:  M Fiandt; W Szybalski; M H Malamy
Journal:  Mol Gen Genet       Date:  1972

3.  Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.

Authors:  H Saedler; B Heiss
Journal:  Mol Gen Genet       Date:  1973-05-09

4.  Mutations caused by the insertion of genetic material into the galactose operon of Escherichia coli.

Authors:  J A Shapiro
Journal:  J Mol Biol       Date:  1969-02-28       Impact factor: 5.469

5.  O0 and strong-polar mutations in the gal operon are insertions.

Authors:  E Jordan; H Saedler; P Starlinger
Journal:  Mol Gen Genet       Date:  1968

6.  Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.

Authors:  P Gay; D Le Coq; M Steinmetz; E Ferrari; J A Hoch
Journal:  J Bacteriol       Date:  1983-03       Impact factor: 3.490

7.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688

8.  Genetic map of the crown gall suppressive IncW plasmid pSa.

Authors:  R C Tait; R C Lundquist; C I Kado
Journal:  Mol Gen Genet       Date:  1982

9.  Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58.

Authors:  T J Close; D Zaitlin; C I Kado
Journal:  Plasmid       Date:  1984-09       Impact factor: 3.466

10.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205
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  216 in total

1.  Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

Authors:  T Asai; C Condon; J Voulgaris; D Zaporojets; B Shen; M Al-Omar; C Squires; C L Squires
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

2.  Efficiency and stability of high molecular weight DNA transformation: an analysis in tomato.

Authors:  A Frary; C M Hamilton
Journal:  Transgenic Res       Date:  2001-04       Impact factor: 2.788

3.  Transduction-mediated transfer of unmarked deletion and point mutations through use of counterselectable suicide vectors.

Authors:  Ho Young Kang; Charles M Dozois; Steven A Tinge; Tae Ho Lee; Roy Curtiss
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

4.  Mutations which alter the elbow region of tRNA2Gly reduce T4 gene 60 translational bypassing efficiency.

Authors:  A J Herr; J F Atkins; R F Gesteland
Journal:  EMBO J       Date:  1999-05-17       Impact factor: 11.598

5.  Molecular evolution of virulence in natural field strains of Xanthomonas campestris pv. vesicatoria.

Authors:  W Gassmann; D Dahlbeck; O Chesnokova; G V Minsavage; J B Jones; B J Staskawicz
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

6.  Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replication.

Authors:  Shiaoching Gong; Xiangdong William Yang; Chenjian Li; Nathaniel Heintz
Journal:  Genome Res       Date:  2002-12       Impact factor: 9.043

7.  Null mutation of HvrA compensates for loss of an essential relA/spoT-like gene in Rhodobacter capsulatus.

Authors:  Shinji Masuda; Carl E Bauer
Journal:  J Bacteriol       Date:  2004-01       Impact factor: 3.490

8.  TmRNA is required for correct timing of DNA replication in Caulobacter crescentus.

Authors:  Kenneth C Keiler; Lucy Shapiro
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

9.  Amino acid contacts between sigma 70 domain 4 and the transcription activators RhaS and RhaR.

Authors:  Jason R Wickstrum; Susan M Egan
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490

10.  The r1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome.

Authors:  Richard Meyer
Journal:  J Bacteriol       Date:  2008-12-12       Impact factor: 3.490
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