Literature DB >> 2158981

Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a "mini" mycobacterium-Escherichia coli shuttle vector.

M G Ranes1, J Rauzier, M Lagranderie, M Gheorghiu, B Gicquel.   

Abstract

Functional domains of pAL5000 were determined by gene disruption and deletion analysis. Of the five plasmid open reading frames (ORFs), ORF1 to ORF5, and a putative origin of replication previously identified (J. Rauzier, J. Moniz-Pereira, and B. Gicquel-Sanzey, Gene 71:315-321), two of the ORFs (ORF3 and ORF4) were deemed dispensable for plasmid replication. A "mini" mycobacterium-Escherichia coli shuttle plasmid applicable for general recombinant DNA studies in mycobacteria was constructed by using the gene for Kanr (Tn903) as a selective marker. Heterologous expression of the gene for Kanr was confirmed by Western blotting (immunoblotting) analysis.

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Year:  1990        PMID： 2158981      PMCID： PMC208931          DOI： 10.1128/jb.172.5.2793-2797.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

Review 1.  The BCG story: lessons from the past and implications for the future.

Authors:  P E Fine
Journal:  Rev Infect Dis       Date:  1989 Mar-Apr

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum.

Authors:  J Rauzier; J Moniz-Pereira; B Gicquel-Sanzey
Journal:  Gene       Date:  1988-11-30       Impact factor: 3.688

4.  A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.

Authors:  J Messing; J Vieira
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

5.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

6.  Restriction endonuclease mapping and cloning of Mycobacterium fortuitum var. fortuitum plasmid pAL5000.

Authors:  A Labidi; H L David; D Roulland-Dussoix
Journal:  Ann Inst Pasteur Microbiol (1985)       Date:  1985 Sep-Oct

7.  Structure of pAL5000, a plasmid from M. fortuitum and its utilization in transformation of mycobacteria.

Authors:  B Gicquel-Sanzey; J Moniz-Pereira; M Gheorghiu; J Rauzier
Journal:  Acta Leprol       Date:  1989

8.  The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation.

Authors:  T McKenzie; T Hoshino; T Tanaka; N Sueoka
Journal:  Plasmid       Date:  1986-03       Impact factor: 3.466

9.  Lysogeny and transformation in mycobacteria: stable expression of foreign genes.

Authors:  S B Snapper; L Lugosi; A Jekkel; R E Melton; T Kieser; B R Bloom; W R Jacobs
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

10.  Introduction of foreign DNA into mycobacteria using a shuttle phasmid.

Authors:  W R Jacobs; M Tuckman; B R Bloom
Journal:  Nature       Date:  1987 Jun 11-17       Impact factor: 49.962
  10 in total
  51 in total

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Authors:  I Méderlé; I Bourguin; D Ensergueix; E Badell; J Moniz-Peireira; B Gicquel; N Winter
Journal:  Infect Immun       Date:  2002-01       Impact factor: 3.441

2.  Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli.

Authors:  A Baulard; C Jourdan; A Mercenier; C Locht
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

Review 3.  Techniques for genetic engineering in mycobacteria. Alternative host strains, DNA-transfer systems and vectors.

Authors:  J Hermans; J A de Bont
Journal:  Antonie Van Leeuwenhoek       Date:  1996-04       Impact factor: 2.271

4.  Isolation by genetic labeling of a new mycobacterial plasmid, pJAZ38, from Mycobacterium fortuitum.

Authors:  J A Gavigan; J A Aínsa; E Pérez; I Otal; C Martín
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

5.  Characterization of Engineered PreQ1 Riboswitches for Inducible Gene Regulation in Mycobacteria.

Authors:  Erik R Van Vlack; Shana Topp; Jessica C Seeliger
Journal:  J Bacteriol       Date:  2017-02-28       Impact factor: 3.490

6.  A novel transposon trap for mycobacteria: isolation and characterization of IS1096.

Authors:  J D Cirillo; R G Barletta; B R Bloom; W R Jacobs
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

7.  Construction of shuttle vectors for genetic manipulation and molecular analysis of mycobacteria.

Authors:  S Jain; D Kaushal; S K DasGupta; A K Tyagi
Journal:  Gene       Date:  1997-04-29       Impact factor: 3.688

8.  Escherichia coli-mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series.

Authors:  J Timm; E M Lim; B Gicquel
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

9.  Optimisation of bioluminescent reporters for use with mycobacteria.

Authors:  Nuria Andreu; Andrea Zelmer; Taryn Fletcher; Paul T Elkington; Theresa H Ward; Jorge Ripoll; Tanya Parish; Gregory J Bancroft; Ulrich Schaible; Brian D Robertson; Siouxsie Wiles
Journal:  PLoS One       Date:  2010-05-24       Impact factor: 3.240

10.  Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.

Authors:  Amit Parikh; Devanand Kumar; Yogesh Chawla; Krishna Kurthkoti; Shazia Khan; Umesh Varshney; Vinay K Nandicoori
Journal:  Appl Environ Microbiol       Date:  2013-01-11       Impact factor: 4.792
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