Literature DB >> 8557383

Epidemiological survey of an outbreak of multiresistant Serratia marcescens by PCR-fingerprinting.

S B Debast1, W J Melchers, A Voss, J A Hoogkamp-Korstanje, J F Meis.   

Abstract

During an outbreak of Serratia marcescens from May to November 1993 43 strains obtained from 27 ICU patients infected or colonized with multiresistant S. marcescens were genotypically characterized with random amplified polymerase chain reaction (RAPD-PCR)-fingerprinting. In addition, 43 epidemiologically unrelated control isolates were selected. PCR-fingerprinting identified ten different genotypes of S. marcescens among the outbreak related strains. One predominant genotype was demonstrated in 21/43 isolates of 11/27 patients. A cluster of this genotype was found in seven/eight patients on the cardiosurgical ICU. The epidemiologically unrelated strains all showed different genotypes as compared to the predominant type. This survey proved RAPD-PCR to be a highly discriminatory and reproducible method for epidemiological studies of S. marcescens strains in nosocomial outbreaks.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 8557383     DOI： 10.1007/bf01716283

Source DB:  PubMed          Journal:  Infection        ISSN： 0300-8126            Impact factor:   3.553


  23 in total

1.  Evaluation of a computer-assisted method of analysing SDS-PAGE protein profiles in tracing a hospital outbreak of Serratia marcescens.

Authors:  A Arzese; G A Botta; G P Gesu; G Schito
Journal:  J Infect       Date:  1988-07       Impact factor: 6.072

2.  Cross-infection with Pseudomonas aeruginosa in a neonatal intensive care unit characterized by polymerase chain reaction fingerprinting.

Authors:  P E Verweij; W B Geven; A van Belkum; J F Meis
Journal:  Pediatr Infect Dis J       Date:  1993-12       Impact factor: 2.129

3.  The use of statistical process control charts in hospital epidemiology.

Authors:  J A Sellick
Journal:  Infect Control Hosp Epidemiol       Date:  1993-11       Impact factor: 3.254

Review 4.  DNA fingerprinting of medically important microorganisms by use of PCR.

Authors:  A van Belkum
Journal:  Clin Microbiol Rev       Date:  1994-04       Impact factor: 26.132

5.  Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification of Serratia marcescens.

Authors:  R Alonso; H M Aucken; J C Perez-Diaz; B D Cookson; F Baquero; T L Pitt
Journal:  Epidemiol Infect       Date:  1993-08       Impact factor: 2.451

6.  Biotyping of Serratia marcescens and its use in epidemiological studies.

Authors:  P A Grimont; F Grimont
Journal:  J Clin Microbiol       Date:  1978-07       Impact factor: 5.948

7.  Bacteriocin (Marcescin) typing of clinical isolates of Serratia marcescens.

Authors:  W H Traub; E A Raymond; T S Startsman
Journal:  Appl Microbiol       Date:  1971-05

8.  Interrepeat fingerprinting of third-generation cephalosporin-resistant Enterobacter cloacae isolated during an outbreak in a neonatal intensive care unit.

Authors:  P E Verweij; A Van Belkum; W J Melchers; A Voss; J A Hoogkamp-Korstanje; J F Meis
Journal:  Infect Control Hosp Epidemiol       Date:  1995-01       Impact factor: 3.254

9.  DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting.

Authors:  N Akopyanz; N O Bukanov; T U Westblom; S Kresovich; D E Berg
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

10.  A hospital outbreak of Serratia marcescens in neurosurgical patients.

Authors:  A M Lewis; J R Stephenson; J Garner; F Afshar; S Tabaqchali
Journal:  Epidemiol Infect       Date:  1989-02       Impact factor: 2.451
View more
  5 in total

1.  PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains.

Authors:  M M Clementino; I de Filippis; C R Nascimento; R Branquinho; C L Rocha; O B Martins
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens.

Authors:  Z Y Shi; P Y Liu; Y J Lau; Y H Lin; B S HU
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

Review 3.  Serratia infections: from military experiments to current practice.

Authors:  Steven D Mahlen
Journal:  Clin Microbiol Rev       Date:  2011-10       Impact factor: 26.132

4.  Application of infrequent-restriction-site PCR to clinical isolates of Acinetobacter baumannii and Serratia marcescens.

Authors:  J H Yoo; J H Choi; W S Shin; D H Huh; Y K Cho; K M Kim; M Y Kim; M W Kang
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

5.  Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry.

Authors:  Bhavya Somalapura Gangadharappa; Sharath Rajashekarappa; Gajanan Sathe
Journal:  Bioimpacts       Date:  2020-03-26
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.