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Literature DB >> 11682499

PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains.

M M Clementino¹, I de Filippis, C R Nascimento, R Branquinho, C L Rocha, O B Martins.

Abstract

PCR analysis of tRNA intergenic spacer (tDNA-PCR) and of the 16S-23S internal transcribed spacer (ITS-PCR) and random amplified polymorphic DNA (RAPD) analysis were evaluated for their usefulness in characterization of Enterobacter cloacae strains isolated from both clinical origins and vaccine microbial contamination. tDNA-PCR presented specific and reproducible patterns for Enterobacter sakazakii ATCC 29004, Enterobacter aerogenes ATCC 13048, and Enterobacter cloacae ATCC 13047 and 23355 that presented the same profile for all 16 E. cloacae isolates, offering an alternative tool for species-level identification. ITS-PCR and RAPD analysis yielded completely different banding patterns for the 20 strains studied, except for E. cloacae strains isolated from different batches of vaccine that exhibited a unique pattern, suggesting contamination by the same strain. The combined use of tDNA-PCR and ITS-PCR in a one-step protocol allows accurate identification and typing of E. cloacae strains a few hours after the colony has been isolated.

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Year: 2001 PMID： 11682499 PMCID： PMC88456 DOI： 10.1128/JCM.39.11.3865-3870.2001

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

33 in total

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8 in total

1. Microbial DNA typing by automated repetitive-sequence-based PCR.

Authors: Mimi Healy; Joe Huong; Traci Bittner; Maricel Lising; Stacie Frye; Sabeen Raza; Robert Schrock; Janet Manry; Alex Renwick; Robert Nieto; Charles Woods; James Versalovic; James R Lupski
Journal: J Clin Microbiol Date: 2005-01 Impact factor: 5.948

2. Diversity of culturable psychrophilic and psychrotrophic anaerobic bacteria isolated from beef abattoirs and their environments.

Authors: G Moschonas; D J Bolton; D A McDowell; J J Sheridan
Journal: Appl Environ Microbiol Date: 2011-04-15 Impact factor: 4.792

PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains.

1. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

2. Enterobacter bacteremia in surgical patients.

3. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli.

Review 4. Enterobacter: an emerging nosocomial pathogen.

Review 5. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region.

6. Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism.

7. Application of tRNA intergenic spacer PCR for identification of Enterococcus species.

8. Genotype analysis of Burkholderia pseudomallei using randomly amplified polymorphic DNA (RAPD): indicative of genetic differences amongst environmental and clinical isolates.

9. Retrospective analysis of plasmid patterns in a study of burn unit outbreaks of infection due to Enterobacter cloacae.

10. Loss of the spacer loop sequence from the rrnB operon in the Escherichia coli K-12 subline that bears the relA1 mutation.

1. Microbial DNA typing by automated repetitive-sequence-based PCR.

2. Diversity of culturable psychrophilic and psychrotrophic anaerobic bacteria isolated from beef abattoirs and their environments.

3. Detection of Polyclonality among Clinical Isolates from Prosthetic Joint Infections.

4. Molecular characterization of Brevibacillus laterosporus and its potential use in biological control.

5. Utility of pyrosequencing in identifying bacteria directly from positive blood culture bottles.

6. Assessment of genetic diversity of Bacillus spp. isolated from eutrophic fish culture pond.

7. Population genetics of the nomenspecies Enterobacter cloacae.

8. Genotypic and phenotypic diversity of Bacillus spp. isolated from steel plant waste.