Literature DB >> 8539617

Functional evidence for indirect recognition of G.U in tRNA(Ala) by alanyl-tRNA synthetase.

K Gabriel1, J Schneider, W H McClain.   

Abstract

The structural features of the G.U wobble pair in Escherichia coli alanine transfer RNA (tRNA(Ala)) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNA(Ala) and mutant tRNAs with G.U substitutions. tRNA(Ala) with G.U, C.A, or G.A gave similar amounts of charged tRNA(Ala) and supported viability of E. coli lacking chromosomal tRNA(Ala) genes. tRNA(Ala) with G.C was inactive. Recognition of G.U by AlaRS thus requires more than the functional groups on G.U in a regular helix and may involve detection of a helical distortion.

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Year:  1996        PMID： 8539617     DOI： 10.1126/science.271.5246.195

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  37 in total

1.  Correlation of deformability at a tRNA recognition site and aminoacylation specificity.

Authors:  K Y Chang; G Varani; S Bhattacharya; H Choi; W H McClain
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

Review 2.  The G x U wobble base pair. A fundamental building block of RNA structure crucial to RNA function in diverse biological systems.

Authors:  G Varani; W H McClain
Journal:  EMBO Rep       Date:  2000-07       Impact factor: 8.807

Review 3.  How translational accuracy influences reading frame maintenance.

Authors:  P J Farabaugh; G R Björk
Journal:  EMBO J       Date:  1999-03-15       Impact factor: 11.598

4.  Modulation of tRNAAla identity by inorganic pyrophosphatase.

Authors:  Alexey D Wolfson; Olke C Uhlenbeck
Journal:  Proc Natl Acad Sci U S A       Date:  2002-04-30       Impact factor: 11.205

5.  Neuronal BC1 RNA structure: evolutionary conversion of a tRNA(Ala) domain into an extended stem-loop structure.

Authors:  T S Rozhdestvensky; A M Kopylov; J Brosius; A Hüttenhofer
Journal:  RNA       Date:  2001-05       Impact factor: 4.942

6.  Crystal structure of acceptor stem of tRNA(Ala) from Escherichia coli shows unique G.U wobble base pair at 1.16 A resolution.

Authors:  U Mueller; H Schübel; M Sprinzl; U Heinemann
Journal:  RNA       Date:  1999-05       Impact factor: 4.942

7.  A yeast knockout strain to discriminate between active and inactive tRNA molecules.

Authors:  Renaud Geslain; Franck Martin; Alain Camasses; Gilbert Eriani
Journal:  Nucleic Acids Res       Date:  2003-08-15       Impact factor: 16.971

8.  The crystal structure at 1.5 angstroms resolution of an RNA octamer duplex containing tandem G.U basepairs.

Authors:  Se Bok Jang; Li-Wei Hung; Mi Suk Jeong; Elizabeth L Holbrook; Xiaoying Chen; Douglas H Turner; Stephen R Holbrook
Journal:  Biophys J       Date:  2006-03-31       Impact factor: 4.033

9.  Crystal structure of an RNA helix recognized by a zinc-finger protein: an 18-bp duplex at 1.6 A resolution.

Authors:  Susana Lima; Jayne Hildenbrand; Andrei Korostelev; Stanley Hattman; Hong Li
Journal:  RNA       Date:  2002-07       Impact factor: 4.942

10.  The modified wobble nucleoside uridine-5-oxyacetic acid in tRNAPro(cmo5UGG) promotes reading of all four proline codons in vivo.

Authors:  S Joakim Nasvall; Peng Chen; Glenn R Bjork
Journal:  RNA       Date:  2004-10       Impact factor: 4.942
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