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Literature DB >> 8535249

Relative effectiveness of various anions on the solubility of acidic Hypoderma lineatum collagenase at pH 7.2.

Abstract

The effects of various anions on decreasing the solubility of acidic Hypoderma lineatum collagenase at pH 7.2 and 18 degrees C were qualitatively defined by replacing the crystallizing agent of known crystallization conditions by various ammonium salts. The solubility curves measured in the presence of the sulfate, phosphate, citrate, and chloride ammonium salts gave the following ranking of anions: HPO4(2-)/H2PO4- > SO4(2-) > citrate 3-/citrate2- >> Cl-. This order is in agreement with the Hofmeister series. In a previous study on the solubility at pH 4.5 of lysozyme, a basic protein, the effectiveness of anions in decreasing the solubility was found to be in the reverse order. This suggests that the effectiveness of anions in the crystallization of proteins is dependent on the net charge of the protein, i.e., depending on whether a basic protein is crystallized at acidic pH or an acidic protein at basic pH.

Entities: Chemical Species

Mesh：

Substances：
Anions
Collagenases

Year: 1995 PMID： 8535249 PMCID： PMC2142976 DOI： 10.1002/pro.5560041018

Source DB: PubMed Journal: Protein Sci ISSN： 0961-8368 Impact factor: 6.725

7 in total

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Authors: M M Ries-Kautt; A F Ducruix
Journal: J Biol Chem Date: 1989-01-15 Impact factor: 5.157

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Journal: Proteins Date: 1993-11

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Journal: Q Rev Biophys Date: 1985-11 Impact factor: 5.318

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Authors: W N Poillon; J F Bertles
Journal: J Biol Chem Date: 1979-05-10 Impact factor: 5.157

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Authors: A Lecroisey; C Boulard; B Keil
Journal: Eur J Biochem Date: 1979-11

7. Acid pH crystallization of the basic protein lysin from the spermatozoa of red abalone (Haliotis rufescens).

Authors: T C Diller; A Shaw; E A Stura; V D Vacquier; C D Stout
Journal: Acta Crystallogr D Biol Crystallogr Date: 1994-07-01

7 in total

8 in total

1. Systematic investigation of protein phase behavior with a microfluidic formulator.

Authors: Carl L Hansen; Morten O A Sommer; Stephen R Quake
Journal: Proc Natl Acad Sci U S A Date: 2004-09-27 Impact factor: 11.205

2. Phase knowledge enables rational screens for protein crystallization.

Authors: Megan J Anderson; Carl L Hansen; Stephen R Quake
Journal: Proc Natl Acad Sci U S A Date: 2006-10-30 Impact factor: 11.205

3. A mechanistic analysis of the increase in the thermal stability of proteins in aqueous carboxylic acid salt solutions.

Authors: J K Kaushik; R Bhat
Journal: Protein Sci Date: 1999-01 Impact factor: 6.725

Relative effectiveness of various anions on the solubility of acidic Hypoderma lineatum collagenase at pH 7.2.

1. Relative effectiveness of various ions on the solubility and crystal growth of lysozyme.

2. Crystallization and preliminary X-ray investigation of barstar, the intracellular inhibitor of barnase.

3. Crystallization and preliminary x-ray diffraction data for the collagenase of Hypoderma lineatum larvae.

Review 4. The Hofmeister effect and the behaviour of water at interfaces.

5. Deoxygenated sickle hemoglobin. Effects of lyotropic salts on its solubility.

6. Chemical and enzymatic characterization of the collagenase from the insect Hypoderma lineatum.

7. Acid pH crystallization of the basic protein lysin from the spermatozoa of red abalone (Haliotis rufescens).

1. Systematic investigation of protein phase behavior with a microfluidic formulator.

2. Phase knowledge enables rational screens for protein crystallization.

3. A mechanistic analysis of the increase in the thermal stability of proteins in aqueous carboxylic acid salt solutions.

4. Ion Specificity and Nonmonotonic Protein Solubility from Salt Entropy.

5. Determination of the phase diagram for soluble and membrane proteins.

6. Urate oxidase purification by salting-in crystallization: towards an alternative to chromatography.

Review 7. Crystallization screening: the influence of history on current practice.

8. Anion-cation contrast of small molecule solvation in salt solutions.