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Literature DB >> 8525379

Precise spatial positioning of chromosomes during prometaphase: evidence for chromosomal order.

R Nagele¹, T Freeman, L McMorrow, H Y Lee.

Abstract

The relative locations of several chromosomes within wheel-shaped prometaphase chromosome rosettes of human fibroblasts and HeLa cells were determined with fluorescence hybridization. Homologs were consistently positioned on opposite sides of the rosette, which suggests that chromosomes are separated into two haploid sets, each derived from one parent. The relative locations of chromosomes on the rosette were mapped by dual hybridizations. The data suggest that the chromosome orders within the two haploid sets are antiparallel. This chromosome arrangement in human cells appears to be both independent of cell type- and species-specific and may influence chromosome topology throughout the cell cycle.

Entities: Species

Mesh：

Substances：
DNA Probes

Year: 1995 PMID： 8525379 DOI： 10.1126/science.270.5243.1831

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

48 in total

Review 1. Higher levels of organization in the interphase nucleus of cycling and differentiated cells.

Authors: A R Leitch
Journal: Microbiol Mol Biol Rev Date: 2000-03 Impact factor: 11.056

2. Arrangements of macro- and microchromosomes in chicken cells.

Authors: F A Habermann; M Cremer; J Walter; G Kreth; J von Hase; K Bauer; J Wienberg; C Cremer; T Cremer; I Solovei
Journal: Chromosome Res Date: 2001 Impact factor: 5.239

3. Non-random radial higher-order chromatin arrangements in nuclei of diploid human cells.

Authors: M Cremer; J von Hase; T Volm; A Brero; G Kreth; J Walter; C Fischer; I Solovei; C Cremer; T Cremer
Journal: Chromosome Res Date: 2001 Impact factor: 5.239

4. Chromosomal painting detects non-random chromosome arrangement in dasyurid marsupial sperm.

Authors: I K Greaves; M Svartman; M Wakefield; D Taggart; A De Leo; M A Ferguson-Smith; W Rens; P C O'Brien; L Voullaire; M Westerman; J A Graves
Journal: Chromosome Res Date: 2001 Impact factor: 5.239

5. The detection of t(14;18) in archival lymph nodes: development of a fluorescence in situ hybridization (FISH)-based method and evaluation by comparison with polymerase chain reaction.

Authors: Sharon L Barrans; Paul A S Evans; Sheila J M O'Connor; Roger G Owen; Gareth J Morgan; Andrew S Jack
Journal: J Mol Diagn Date: 2003-08 Impact factor: 5.568

Precise spatial positioning of chromosomes during prometaphase: evidence for chromosomal order.

Review 1. Higher levels of organization in the interphase nucleus of cycling and differentiated cells.

2. Arrangements of macro- and microchromosomes in chicken cells.

3. Non-random radial higher-order chromatin arrangements in nuclei of diploid human cells.

4. Chromosomal painting detects non-random chromosome arrangement in dasyurid marsupial sperm.

5. The detection of t(14;18) in archival lymph nodes: development of a fluorescence in situ hybridization (FISH)-based method and evaluation by comparison with polymerase chain reaction.

6. The 3D structure of human chromosomes in cell nuclei.

7. The Caenorhabditis elegans kinetochore reorganizes at prometaphase and in response to checkpoint stimuli.

8. Non-random positioning of chromosomes in human sperm nuclei.

9. Pluripotency of a polyploid H1 (ES) cell system without leukaemia inhibitory factor.

10. Dynamics of relative chromosome position during the cell cycle.