Literature DB >> 8506278

Evidence for lack of DNA photoreactivating enzyme in humans.

Y F Li1, S T Kim, A Sancar.   

Abstract

Photoreactivating enzyme (DNA photolyase; deoxyribocyclobutadipyrimidine pyrimidine-lyase, EC 4.1.99.3) repairs UV damage to DNA by utilizing the energy of near-UV/visible light to split pyrimidine dimers into monomers. The enzyme is widespread in nature but is absent in certain species in a seemingly unpredictable manner. Its presence in humans has been a source of considerable controversy. To help resolve the issue we used a very specific and sensitive assay to compare photoreactivation activity in human, rattlesnake, yeast, and Escherichia coli cells. Photolyase was easily detectable in E. coli, yeast, and rattlesnake cell-free extracts but none was detected in cell-free extracts from HeLa cells or human white blood cells with an assay capable of detecting 10 molecules per cell. We conclude that humans most likely do not have DNA photolyase.

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Year:  1993        PMID： 8506278      PMCID： PMC46516          DOI： 10.1073/pnas.90.10.4389

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  39 in total

1.  Detection of photorepair of UV-induced thymine dimers in human epidermis by immunofluorescence microscopy.

Authors:  L Roza; F R De Gruijl; J B Bergen Henegouwen; K Guikers; H Van Weelden; G P Van Der Schans; R A Baan
Journal:  J Invest Dermatol       Date:  1991-06       Impact factor: 8.551

2.  Photoreactivation of herpes simplex virus in human fibroblasts.

Authors:  E K Wagner; M Rice; B M Sutherland
Journal:  Nature       Date:  1975-04-17       Impact factor: 49.962

3.  DNA photoreactivating enzyme from the cyanobacterium Anacystis nidulans.

Authors:  A P Eker; P Kooiman; J K Hessels; A Yasui
Journal:  J Biol Chem       Date:  1990-05-15       Impact factor: 5.157

4.  Photoreactivating-enzyme activity in metazoa.

Authors:  J S Cook; J R McGrath
Journal:  Proc Natl Acad Sci U S A       Date:  1967-10       Impact factor: 11.205

5.  Characterization of antibodies specific for UV-damaged DNA by ELISA.

Authors:  G Eggset; G Volden; H Krokan
Journal:  Photochem Photobiol       Date:  1987-04       Impact factor: 3.421

6.  Culture conditions affect photoreactivating enzyme levels in human fibroblasts.

Authors:  B M Sutherland; R Oliver
Journal:  Biochim Biophys Acta       Date:  1976-09-06

7.  Damage and repair in mammalian cells after exposure to non-ionizing radiations. III. Ultraviolet and visible light irradiation of cells of placental mammals, including humans, and determination of photorepairable damage in vitro.

Authors:  H Harm
Journal:  Mutat Res       Date:  1980-01       Impact factor: 2.433

8.  A constitutive damage-specific DNA-binding protein is synthesized at higher levels in UV-irradiated primate cells.

Authors:  S Hirschfeld; A S Levine; K Ozato; M Protić
Journal:  Mol Cell Biol       Date:  1990-05       Impact factor: 4.272

9.  Photorepair of pyrimidine dimers in human skin in vivo.

Authors:  S M D'Ambrosio; J W Whetstone; L Slazinski; E Lowney
Journal:  Photochem Photobiol       Date:  1981-10       Impact factor: 3.421

10.  Human nucleotide excision repair in vitro: repair of pyrimidine dimers, psoralen and cisplatin adducts by HeLa cell-free extract.

Authors:  I Husain; W Carlton; A Sancar
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971
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  22 in total

1.  Cloning and characterization of a class II DNA photolyase from Chlamydomonas.

Authors:  J L Petersen; D W Lang; G D Small
Journal:  Plant Mol Biol       Date:  1999-08       Impact factor: 4.076

2.  A phylogenomic study of DNA repair genes, proteins, and processes.

Authors:  J A Eisen; P C Hanawalt
Journal:  Mutat Res       Date:  1999-12-07       Impact factor: 2.433

3.  Profile of Tomas Lindahl, Paul Modrich, and Aziz Sancar, 2015 Nobel Laureates in Chemistry.

Authors:  James E Cleaver
Journal:  Proc Natl Acad Sci U S A       Date:  2015-12-29       Impact factor: 11.205

4.  Profile of Aziz Sancar.

Authors:  Nick Zagorski
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-01       Impact factor: 11.205

5.  An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis.

Authors:  M Ahmad; J A Jarillo; L J Klimczak; L G Landry; T Peng; R L Last; A R Cashmore
Journal:  Plant Cell       Date:  1997-02       Impact factor: 11.277

6.  Bacterial cryptochrome and photolyase: characterization of two photolyase-like genes of Synechocystis sp. PCC6803.

Authors:  K Hitomi; K Okamoto; H Daiyasu; H Miyashita; S Iwai; H Toh; M Ishiura; T Todo
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

7.  A novel role for DNA photolyase: binding to DNA damaged by drugs is associated with enhanced cytotoxicity in Saccharomyces cerevisiae.

Authors:  M E Fox; B J Feldman; G Chu
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

8.  Transfection of pseudouridine-modified mRNA encoding CPD-photolyase leads to repair of DNA damage in human keratinocytes: a new approach with future therapeutic potential.

Authors:  Gábor Boros; Edit Miko; Hiromi Muramatsu; Drew Weissman; Eszter Emri; Dávid Rózsa; Georgina Nagy; Attila Juhász; István Juhász; Gijsbertus van der Horst; Irén Horkay; Éva Remenyik; Katalin Karikó; Gabriella Emri
Journal:  J Photochem Photobiol B       Date:  2013-10-11       Impact factor: 6.252

9.  Little or No Repair of Cyclobutyl Pyrimidine Dimers Is Observed in the Organellar Genomes of the Young Arabidopsis Seedling.

Authors:  J. J. Chen; C. Z. Jiang; A. B. Britt
Journal:  Plant Physiol       Date:  1996-05       Impact factor: 8.340

10.  Identification of two amino acids in the C-terminal domain of mouse CRY2 essential for PER2 interaction.

Authors:  Natali Ozber; Ibrahim Baris; Gulnaz Tatlici; Ibrahim Gur; Seda Kilinc; Evrim B Unal; Ibrahim H Kavakli
Journal:  BMC Mol Biol       Date:  2010-09-14       Impact factor: 2.946
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