| Literature DB >> 8506125 |
Abstract
We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 DNA ligase. We find that the ensemble of selected sequences ligates about 50 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly many of the selected sequences failed to produce a match at or close to the ligation junction. None of the 20 selected oligomers that we sequenced produced a match two bases upstream from the ligation junction.Keywords: NASA Discipline Exobiology; NASA Discipline Number 52-20; NASA Discipline Number 93-10; NASA Program Exobiology; NASA Program NSCORT; Non-NASA Center
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Year: 1993 PMID： 8506125 PMCID： PMC309522 DOI： 10.1093/nar/21.10.2287
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971