Literature DB >> 3413476

A ligase-mediated gene detection technique.

U Landegren1, R Kaiser, J Sanders, L Hood.   

Abstract

An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3413476     DOI： 10.1126/science.3413476

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  178 in total

1.  RNA-templated DNA ligation for transcript analysis.

Authors:  M Nilsson; D O Antson; G Barbany; U Landegren
Journal:  Nucleic Acids Res       Date:  2001-01-15       Impact factor: 16.971

Review 2.  Automated mutation analysis.

Authors:  D Ravine
Journal:  J Inherit Metab Dis       Date:  1999-06       Impact factor: 4.982

3.  A microsphere-based assay for multiplexed single nucleotide polymorphism analysis using single base chain extension.

Authors:  J Chen; M A Iannone; M S Li; J D Taylor; P Rivers; A J Nelsen; K A Slentz-Kesler; A Roses; M P Weiner
Journal:  Genome Res       Date:  2000-04       Impact factor: 9.043

Review 4.  Molecular techniques in biomedical sciences: a new era in diagnosis of infectious diseases.

Authors:  S Chandwani; A Kaul
Journal:  Indian J Pediatr       Date:  1995 Jan-Feb       Impact factor: 1.967

5.  PCR-generated padlock probes detect single nucleotide variation in genomic DNA.

Authors:  D O Antson; A Isaksson; U Landegren; M Nilsson
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

6.  L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs).

Authors:  X Qi; S Bakht; K M Devos; M D Gale; A Osbourn
Journal:  Nucleic Acids Res       Date:  2001-11-15       Impact factor: 16.971

7.  Genetic screening using the colour change of a PNA-DNA hybrid-binding cyanine dye.

Authors:  L Marcus Wilhelmsson; Bengt Nordén; Kaushik Mukherjee; Maria T Dulay; Richard N Zare
Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

8.  Competitive amplification and unspecific amplification in polymerase chain reaction with confronting two-pair primers.

Authors:  Nobuyuki Hamajima; Toshiko Saito; Keitaro Matsuo; Kazuo Tajima
Journal:  J Mol Diagn       Date:  2002-05       Impact factor: 5.568

9.  Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing.

Authors:  A Alderborn; A Kristofferson; U Hammerling
Journal:  Genome Res       Date:  2000-08       Impact factor: 9.043

10.  Detection of allelic imbalance in the gene expression of hMSH2 or RB1 in lymphocytes from pedigrees of hereditary, nonpolyposis, colorectal cancer and retinoblastoma by an RNA difference plot.

Authors:  Yoshinori Murakami; Kana Isogai; Hiroyuki Tomita; Mika Sakurai-Yageta; Tomoko Maruyama; Akio Hidaka; Kiyoshi Nose; Kokichi Sugano; Akihiro Kaneko
Journal:  J Hum Genet       Date:  2004-10-08       Impact factor: 3.172
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.