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Literature DB >> 7536299

All you wanted to know about SELEX.

Abstract

In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

Entities: Chemical

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Year: 1994 PMID： 7536299 DOI： 10.1007/bf00996358

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

45 in total

1. In vitro selection of RNAs that undergo autolytic cleavage with Pb2+.

Authors: T Pan; O C Uhlenbeck
Journal: Biochemistry Date: 1992-04-28 Impact factor: 3.162

2. Selection of a ribozyme that functions as a superior template in a self-copying reaction.

Authors: R Green; J W Szostak
Journal: Science Date: 1992-12-18 Impact factor: 47.728

3. Directed evolution of an RNA enzyme.

Authors: A A Beaudry; G F Joyce
Journal: Science Date: 1992-07-31 Impact factor: 47.728

4. Randomization of genes by PCR mutagenesis.

Authors: R C Cadwell; G F Joyce
Journal: PCR Methods Appl Date: 1992-08

5. HIV-1 regulator of virion expression (Rev) protein binds to an RNA stem-loop structure located within the Rev response element region.

Authors: S Heaphy; C Dingwall; I Ernberg; M J Gait; S M Green; J Karn; A D Lowe; M Singh; M A Skinner
Journal: Cell Date: 1990-02-23 Impact factor: 41.582

6. The application of a modified nucleotide in aptamer selection: novel thrombin aptamers containing 5-(1-pentynyl)-2'-deoxyuridine.

Authors: J A Latham; R Johnson; J J Toole
Journal: Nucleic Acids Res Date: 1994-07-25 Impact factor: 16.971

7. Selection of high-affinity RNA ligands to reverse transcriptase: inhibition of cDNA synthesis and RNase H activity.

Authors: H Chen; L Gold
Journal: Biochemistry Date: 1994-07-26 Impact factor: 3.162

8. Sequence-specific RNA binding by the HIV-1 Rev protein.

Authors: M L Zapp; M R Green
Journal: Nature Date: 1989-12-07 Impact factor: 49.962

9. In vitro selection of RNA aptamers specific for cyanocobalamin.

Authors: J R Lorsch; J W Szostak
Journal: Biochemistry Date: 1994-02-01 Impact factor: 3.162

10. Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.

Authors: F Michel; A Jacquier; B Dujon
Journal: Biochimie Date: 1982-10 Impact factor: 4.079

66 in total

1. In vitro selection of RNA aptamers that bind special elongation factor SelB, a protein with multiple RNA-binding sites, reveals one major interaction domain at the carboxyl terminus.

Authors: S J Klug; A Hüttenhofer; M Famulok
Journal: RNA Date: 1999-09 Impact factor: 4.942

2. Anti-Rex aptamers as mimics of the Rex-binding element.

Authors: S Baskerville; M Zapp; A D Ellington
Journal: J Virol Date: 1999-06 Impact factor: 5.103

3. A novel assay to determine the sequence preference and affinity of DNA minor groove binding compounds.

Authors: Rita Thomas; Carolyn Gonzalez; Christopher Roberts; Janos Botyanszki; Lillian Lou; Emil F Michelotti
Journal: Nucleic Acids Res Date: 2004-01-12 Impact factor: 16.971

4. Aptamer database.

Authors: Jennifer F Lee; Jay R Hesselberth; Lauren Ancel Meyers; Andrew D Ellington
Journal: Nucleic Acids Res Date: 2004-01-01 Impact factor: 16.971

5. Identification of cellular mRNA targets for RNA-binding protein Sam68.

Authors: Michiyasu Itoh; Izumi Haga; Qing-Hua Li; Jun-ichi Fujisawa
Journal: Nucleic Acids Res Date: 2002-12-15 Impact factor: 16.971

6. Determination of target sequence bound by PapX, repressor of bacterial motility, in flhD promoter using systematic evolution of ligands by exponential enrichment (SELEX) and high throughput sequencing.

Authors: Daniel J Reiss; Harry L T Mobley
Journal: J Biol Chem Date: 2011-10-28 Impact factor: 5.157