Literature DB >> 8497047

Hybrid human immunodeficiency virus Gag particles as an antigen carrier system: induction of cytotoxic T-cell and humoral responses by a Gag:V3 fusion.

J C Griffiths1, S J Harris, G T Layton, E L Berrie, T J French, N R Burns, S E Adams, A J Kingsman.   

Abstract

In attempts to increase the immunogenicity of recombinant antigens, a number of particulate antigen presentation systems have been developed. In this study, we used human immunodeficiency virus Gag particles as carriers for the human immunodeficiency virus envelope V3 region. Gag:V3 fusion proteins were expressed from baculovirus expression vectors; they migrated to the insect cell membrane and budded from the cells as hybrid particles. An immunization study carried out with rats showed that the particles elicited a strong anti-Gag antibody response and a weak antibody response to the V3 region. A strong anti-V3 cytolytic T-cell response was elicited in immunized mice. These data show that retroviral Gag particles can be used as antigen presentation vehicles.

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Year:  1993        PMID： 8497047      PMCID： PMC237658          DOI： 10.1128/JVI.67.6.3191-3198.1993

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  52 in total

1.  Cytotoxic T-cell recognition of HIV proteins and peptides.

Authors:  D F Nixon; A J McMichael
Journal:  AIDS       Date:  1991-09       Impact factor: 4.177

2.  HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. Fine specificity of the gag-specific response defined by using unstimulated peripheral blood mononuclear cells and cloned effector cells.

Authors:  R P Johnson; A Trocha; L Yang; G P Mazzara; D L Panicali; T M Buchanan; B D Walker
Journal:  J Immunol       Date:  1991-09-01       Impact factor: 5.422

3.  Priming of anti-human immunodeficiency virus (HIV) CD8+ cytotoxic T cells in vivo by carrier-free HIV synthetic peptides.

Authors:  M K Hart; K J Weinhold; R M Scearce; E M Washburn; C A Clark; T J Palker; B F Haynes
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

4.  Functional domains of HIV-1 gag-polyprotein expressed in baculovirus-infected cells.

Authors:  M Royer; M Cerutti; B Gay; S S Hong; G Devauchelle; P Boulanger
Journal:  Virology       Date:  1991-09       Impact factor: 3.616

5.  Liposome-encapsulated antigens engender lysosomal processing for class II MHC presentation and cytosolic processing for class I presentation.

Authors:  C V Harding; D S Collins; O Kanagawa; E R Unanue
Journal:  J Immunol       Date:  1991-11-01       Impact factor: 5.422

6.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

7.  HIV-specific humoral and cellular immunity in rabbits vaccinated with recombinant human immunodeficiency virus-like gag-env particles.

Authors:  O K Haffar; M D Smithgall; P A Moran; B M Travis; J M Zarling; S L Hu
Journal:  Virology       Date:  1991-08       Impact factor: 3.616

8.  Cross-neutralizing antibodies to HIV-1 in mice after immunization with gp160 iscoms. Dissection of the immune response.

Authors:  L Akerblom; P Nara; N Dunlop; B Morein
Journal:  AIDS Res Hum Retroviruses       Date:  1991-07       Impact factor: 2.205

9.  Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS.

Authors:  J Schüpbach; M Popovic; R V Gilden; M A Gonda; M G Sarngadharan; R C Gallo
Journal:  Science       Date:  1984-05-04       Impact factor: 47.728

10.  Precise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes.

Authors:  E G Pamer; J T Harty; M J Bevan
Journal:  Nature       Date:  1991-10-31       Impact factor: 49.962
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  18 in total

1.  In vivo induction of a high-avidity, high-frequency cytotoxic T-lymphocyte response is associated with antiviral protective immunity.

Authors:  C Sedlik; G Dadaglio; M F Saron; E Deriaud; M Rojas; S I Casal; C Leclerc
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

2.  Formation of polyomavirus-like particles with different VP1 molecules that bind the urokinase plasminogen activator receptor.

Authors:  Young C Shin; William R Folk
Journal:  J Virol       Date:  2003-11       Impact factor: 5.103

3.  Induction of single and dual cytotoxic T-lymphocyte responses to viral proteins in mice using recombinant hybrid Ty-virus-like particles.

Authors:  G T Layton; S J Harris; J Myhan; D West; F Gotch; M Hill-Perkins; J S Cole; N Meyers; S Woodrow; T J French; S E Adams; A J Kingsman
Journal:  Immunology       Date:  1996-02       Impact factor: 7.397

4.  Recombinant parvovirus-like particles as an antigen carrier: a novel nonreplicative exogenous antigen to elicit protective antiviral cytotoxic T cells.

Authors:  C Sedlik; M Saron; J Sarraseca; I Casal; C Leclerc
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-08       Impact factor: 11.205

5.  Heterogeneity of envelope molecules expressed on primary human immunodeficiency virus type 1 particles as probed by the binding of neutralizing and nonneutralizing antibodies.

Authors:  Pascal Poignard; Maxime Moulard; Edwin Golez; Veronique Vivona; Michael Franti; Sara Venturini; Meng Wang; Paul W H I Parren; Dennis R Burton
Journal:  J Virol       Date:  2003-01       Impact factor: 5.103

6.  Contribution of virus-like particles to the immunogenicity of human immunodeficiency virus type 1 Gag-derived vaccines in mice.

Authors:  S B Justin Wong; Robert F Siliciano
Journal:  J Virol       Date:  2005-02       Impact factor: 5.103

7.  Expression and characterization of virus-like particles containing rubella virus structural proteins.

Authors:  Z Qiu; D Ou; T C Hobman; S Gillam
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

8.  Antibody and cytotoxic T-cell responses to soluble hepatitis B virus (HBV) S antigen in mice: implication for the pathogenesis of HBV-induced hepatitis.

Authors:  R Schirmbeck; K Melber; T Mertens; J Reimann
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103

9.  Enhanced presentation of major histocompatibility complex class I-restricted human immunodeficiency virus type 1 (HIV-1) Gag-specific epitopes after DNA immunization with vectors coding for vesicular stomatitis virus glycoprotein-pseudotyped HIV-1 Gag particles.

Authors:  D Marsac; D Loirat; C Petit; O Schwartz; M-L Michel
Journal:  J Virol       Date:  2002-08       Impact factor: 5.103

Review 10.  HIV preventive vaccines. Progress to date.

Authors:  J Esparza; S Osmanov; W L Heyward
Journal:  Drugs       Date:  1995-11       Impact factor: 9.546
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