Literature DB >> 8189549

Expression and characterization of virus-like particles containing rubella virus structural proteins.

Z Qiu1, D Ou, T C Hobman, S Gillam.   

Abstract

Rubella virus (RV) virions contain two envelope glycoproteins (E1 and E2) and a capsid protein (C). Noninfectious RV-like particles (VLPs) containing three structural proteins were expressed in a BHK cell line (BHK-24S) by using an inducible promoter. These VLPs were found to resemble RV virons in terms of their size, their morphology, and some biological activities. In immunoblotting studies, VLPs were found to bind similarly to native RV virions with 10 of a panel of 12 RV-specific murine monoclonal antibodies. Immunization of mice with VLPs induced specific antibody responses against RV structural proteins as well as virus-neutralizing and hemagglutination-inhibiting antibodies. After immunization of mice with VLPs, in vitro challenge of isolated lymphocytes with inactivated RV and individual RV structural proteins stimulated proliferation. Our data suggest the possibility of using VLPs as immunogens for serodiagnostic assays and RV vaccines.

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Year:  1994        PMID： 8189549      PMCID： PMC236923     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  39 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

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Journal:  J Virol       Date:  1972-01       Impact factor: 5.103

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Journal:  Intervirology       Date:  1982       Impact factor: 1.763

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Authors:  M N Waxham; J S Wolinsky
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616

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Authors:  D Ou; P Chong; A J Tingle; S Gillam
Journal:  J Med Virol       Date:  1993-07       Impact factor: 2.327

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Authors:  J K Chantler; D K Ford; A J Tingle
Journal:  Lancet       Date:  1982-06-12       Impact factor: 79.321

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Authors:  H Liebhaber
Journal:  J Immunol       Date:  1970-04       Impact factor: 5.422

8.  Human T- and B-cell epitopes of E1 glycoprotein of rubella virus.

Authors:  H Chaye; D Ou; P Chong; S Gillam
Journal:  J Clin Immunol       Date:  1993-03       Impact factor: 8.317

9.  Chimeric influenza virus induces neutralizing antibodies and cytotoxic T cells against human immunodeficiency virus type 1.

Authors:  S Li; V Polonis; H Isobe; H Zaghouani; R Guinea; T Moran; C Bona; P Palese
Journal:  J Virol       Date:  1993-11       Impact factor: 5.103

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Authors:  C M Gorman; L F Moffat; B H Howard
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272
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  20 in total

1.  Role of rubella virus glycoprotein domains in assembly of virus-like particles.

Authors:  M Garbutt; L M Law; H Chan; T C Hobman
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

Review 2.  Rubella virus replication and links to teratogenicity.

Authors:  J Y Lee; D S Bowden
Journal:  Clin Microbiol Rev       Date:  2000-10       Impact factor: 26.132

3.  A single-amino-acid substitution of a tyrosine residue in the rubella virus E1 cytoplasmic domain blocks virus release.

Authors:  J Yao; S Gillam
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

4.  Generation and characterization of a mammalian cell line continuously expressing Japanese encephalitis virus subviral particles.

Authors:  E Konishi; A Fujii; P W Mason
Journal:  J Virol       Date:  2001-03       Impact factor: 5.103

5.  Rubella virus-like replicon particles: analysis of encapsidation determinants and non-structural roles of capsid protein in early post-entry replication.

Authors:  Claudia Claus; Wen-Pin Tzeng; U G Liebert; Teryl K Frey
Journal:  J Gen Virol       Date:  2011-11-23       Impact factor: 3.891

6.  The two major envelope proteins of equine arteritis virus associate into disulfide-linked heterodimers.

Authors:  A A de Vries; S M Post; M J Raamsman; M C Horzinek; P J Rottier
Journal:  J Virol       Date:  1995-08       Impact factor: 5.103

7.  Mutational analysis, using a full-length rubella virus cDNA clone, of rubella virus E1 transmembrane and cytoplasmic domains required for virus release.

Authors:  J Yao; S Gillam
Journal:  J Virol       Date:  1999-06       Impact factor: 5.103

8.  Rubella virus capsid protein structure and its role in virus assembly and infection.

Authors:  Vidya Mangala Prasad; Steven D Willows; Andrei Fokine; Anthony J Battisti; Siyang Sun; Pavel Plevka; Tom C Hobman; Michael G Rossmann
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-26       Impact factor: 11.205

Review 9.  Molecular and Structural Insights into the Life Cycle of Rubella Virus.

Authors:  Pratyush Kumar Das; Margaret Kielian
Journal:  J Virol       Date:  2021-02-24       Impact factor: 5.103

10.  Effects of mutations in the rubella virus E1 glycoprotein on E1-E2 interaction and membrane fusion activity.

Authors:  D Yang; D Hwang; Z Qiu; S Gillam
Journal:  J Virol       Date:  1998-11       Impact factor: 5.103
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