Literature DB >> 8481013

Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus.

S Paavilainen1, J Hellman, T Korpela.   

Abstract

An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated M(r) of 51,303. The enzyme exhibited from 45 to 66% identity with five bacterial beta-glucosidases.

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Year:  1993        PMID： 8481013      PMCID： PMC202212          DOI： 10.1128/aem.59.3.927-932.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  25 in total

Review 1.  Molecular biology of cellulose degradation.

Authors:  P Béguin
Journal:  Annu Rev Microbiol       Date:  1990       Impact factor: 15.500

2.  Sequences and homology analysis of two genes encoding beta-glucosidases from Bacillus polymyxa.

Authors:  L González-Candelas; D Ramón; J Polaina
Journal:  Gene       Date:  1990-10-30       Impact factor: 3.688

3.  Cloning, sequencing and analysis of expression of a Butyrivibrio fibrisolvens gene encoding a beta-glucosidase.

Authors:  L L Lin; E Rumbak; H Zappe; J A Thomson; D R Woods
Journal:  J Gen Microbiol       Date:  1990-08

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene.

Authors:  M V Cubellis; C Rozzo; P Montecucchi; M Rossi
Journal:  Gene       Date:  1990-09-28       Impact factor: 3.688

6.  Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis.

Authors:  W M De Vos; M J Gasson
Journal:  J Gen Microbiol       Date:  1989-07

7.  Purification and characterization of beta-glucosidase of Alcaligenes faecalis.

Authors:  Y W Han; V R Srinivasan
Journal:  J Bacteriol       Date:  1969-12       Impact factor: 3.490

8.  Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule.

Authors:  L A Castle; K D Smith; R O Morris
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

9.  Structure of the beta-glucosidase gene bglA of Clostridium thermocellum. Sequence analysis reveals a superfamily of cellulases and beta-glycosidases including human lactase/phlorizin hydrolase.

Authors:  F Gräbnitz; M Seiss; K P Rücknagel; W L Staudenbauer
Journal:  Eur J Biochem       Date:  1991-09-01

10.  Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid.

Authors:  P H Hirel; M J Schmitter; P Dessen; G Fayat; S Blanquet
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205
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  14 in total

1.  Structural basis for thermostability of beta-glycosidase from the thermophilic eubacterium Thermus nonproteolyticus HG102.

Authors:  Xinquan Wang; Xiangyuan He; Shoujun Yang; Xiaomin An; Wenrui Chang; Dongcai Liang
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

2.  Genes encoding two different beta-glucosidases of Thermoanaerobacter brockii are clustered in a common operon.

Authors:  R Breves; K Bronnenmeier; N Wild; F Lottspeich; W L Staudenbauer; J Hofemeister
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

3.  Detection of beta-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate.

Authors:  K S Kwon; J Lee; H G Kang; Y C Hah
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792

4.  Production, Purification, and Properties of a Thermostable beta-Glucosidase from a Color Variant Strain of Aureobasidium pullulans.

Authors:  B C Saha; S N Freer; R J Bothast
Journal:  Appl Environ Microbiol       Date:  1994-10       Impact factor: 4.792

5.  Employing a biochemical protecting group for a sustainable indigo dyeing strategy.

Authors:  Tammy M Hsu; Ditte H Welner; Zachary N Russ; Bernardo Cervantes; Ramya L Prathuri; Paul D Adams; John E Dueber
Journal:  Nat Chem Biol       Date:  2018-01-08       Impact factor: 15.040

6.  Characterization of two new glycosyl hydrolases from the lactic acid bacterium Carnobacterium piscicola strain BA.

Authors:  J Coombs; J E Brenchley
Journal:  Appl Environ Microbiol       Date:  2001-11       Impact factor: 4.792

7.  Enzymatic properties and intracellular localization of the novel Trichoderma reesei beta-glucosidase BGLII (cel1A).

Authors:  Markku Saloheimo; Juha Kuja-Panula; Erkko Ylösmäki; Michael Ward; Merja Penttilä
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

8.  Properties of a recombinant beta-glucosidase from polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis.

Authors:  Xin-Liang Li; Lars G Ljungdahl; Eduardo A Ximenes; Huizhong Chen; Carlos R Felix; Michael A Cotta; Bruce S Dien
Journal:  Appl Biochem Biotechnol       Date:  2004       Impact factor: 2.926

9.  Construction and characterization of a chimeric beta-glucosidase.

Authors:  A Singh; K Hayashi; T T Hoa; Y Kashiwagi; K Tokuyasu
Journal:  Biochem J       Date:  1995-02-01       Impact factor: 3.857

10.  Production, purification, and characterization of a highly glucose-tolerant novel beta-glucosidase from Candida peltata.

Authors:  B C Saha; R J Bothast
Journal:  Appl Environ Microbiol       Date:  1996-09       Impact factor: 4.792
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