Literature DB >> 16349468

Detection of beta-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate.

K S Kwon1, J Lee, H G Kang, Y C Hah.   

Abstract

beta-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by beta-glucosidase action reacts with ferric ion to produce a black band, corresponding to the beta-glucosidase, against the transparent background.

Entities:  

Year:  1994        PMID： 16349468      PMCID： PMC202023          DOI： 10.1128/aem.60.12.4584-4586.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  5 in total

Review 1.  Staining for enzymatic activity after gel electrophoresis, I.

Authors:  O Gabriel; D M Gersten
Journal:  Anal Biochem       Date:  1992-05-15       Impact factor: 3.365

2.  Azo Dye Colony Staining Technique To Detect Fungal Mutants Lacking beta-Glucosidase Activity.

Authors:  J F Rissler
Journal:  Appl Environ Microbiol       Date:  1983-01       Impact factor: 4.792

3.  Characterization of beta-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietinum L.) cell suspension cultures.

Authors:  W Hösel; E Surholt; E Borgmann
Journal:  Eur J Biochem       Date:  1978-03-15

4.  Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels.

Authors:  A Blank; R H Sugiyama; C A Dekker
Journal:  Anal Biochem       Date:  1982-03-01       Impact factor: 3.365

5.  Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus.

Authors:  S Paavilainen; J Hellman; T Korpela
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792
  5 in total
  12 in total

1.  Microbiome of fungus-growing termites: a new reservoir for lignocellulase genes.

Authors:  Ning Liu; Xing Yan; Meiling Zhang; Lei Xie; Qian Wang; Yongping Huang; Xuguo Zhou; Shengyue Wang; Zhihua Zhou
Journal:  Appl Environ Microbiol       Date:  2010-11-05       Impact factor: 4.792

2.  Higher Activities of Defense-Associated Enzymes may Contribute to Greater Resistance of Manchurian Ash to Emerald Ash Borer Than A closely Related and Susceptible Congener.

Authors:  Chad M Rigsby; Daniel A Herms; Pierluigi Bonello; Don Cipollini
Journal:  J Chem Ecol       Date:  2016-08-02       Impact factor: 2.626

3.  Properties of an intracellular beta-glucosidase purified from the cellobiose-fermenting yeast Candida wickerhamii.

Authors:  C D Skory; S N Freer; R J Bothast
Journal:  Appl Microbiol Biotechnol       Date:  1996-11       Impact factor: 4.813

4.  Production of β-glucosidase from okara fermentation using Kluyveromyces marxianus.

Authors:  Min Su; Yang Hu; Yang Cui; Yuhua Wang; Hansong Yu; Junmei Liu; Weichang Dai; Chunhong Piao
Journal:  J Food Sci Technol       Date:  2020-07-08       Impact factor: 2.701

5.  The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014.

Authors:  Hak Joo Choi; Eun A Kim; Dong Hee Kim; Kwang-Soo Shin
Journal:  Mycobiology       Date:  2014-09-30       Impact factor: 1.858

6.  Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation.

Authors:  Vanessa de Cássia Teixeira da Silva; Amanda Lais de Souza Coto; Rafael de Carvalho Souza; Marcello Bertoldi Sanchez Neves; Eleni Gomes; Gustavo Orlando Bonilla-Rodriguez
Journal:  Biochem Res Int       Date:  2016-05-08

7.  Functional Studies of β-Glucosidases of Cytophaga hutchinsonii and Their Effects on Cellulose Degradation.

Authors:  Xinfeng Bai; Xifeng Wang; Sen Wang; Xiaofei Ji; Zhiwei Guan; Weican Zhang; Xuemei Lu
Journal:  Front Microbiol       Date:  2017-02-02       Impact factor: 5.640

8.  A novel method for screening beta-glucosidase inhibitors.

Authors:  Sony Pandey; Ayinampudi Sree; Soumya S Dash; Dipti P Sethi
Journal:  BMC Microbiol       Date:  2013-03-08       Impact factor: 3.605

9.  Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows.

Authors:  Lingling Wang; Ayat Hatem; Umit V Catalyurek; Mark Morrison; Zhongtang Yu
Journal:  PLoS One       Date:  2013-11-05       Impact factor: 3.240

10.  Untreated Chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases.

Authors:  Marcoaurélio Almenara Rodrigues; Ricardo Sposina Sobral Teixeira; Viridiana Santana Ferreira-Leitão; Elba Pinto da Silva Bon
Journal:  Biotechnol Biofuels       Date:  2015-02-19       Impact factor: 6.040
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