Literature DB >> 8464356

A thermospray mass spectrometric assay for Fe-induced 4-hydroxynonenal in tissues.

W J Blanchflower1, D M Walsh, S Kennedy, D G Kennedy.   

Abstract

A method is presented for the determination of 4-hydroxynonenal (HNE) in tissue homogenates following in vitro lipid peroxidation induced by iron (Fe++). HNE is measured as the pentafluorobenzyl oxime derivative using liquid chromatography thermospray mass spectrometry. In vitro metabolism of HNE via the glutathione/glutathione-S-transferase pathway was inhibited using iodoacetic and iodobenzoic acids. The assay has been used as an indicator of the peroxidizability of tissue samples from animals both adequate in and depleted of alpha-tocopherol. The concentrations of HNE produced in tissues taken from animals depleted of alpha-tocopherol were found to be up to 8 times higher than those taken from animals supplemented with alpha-tocopherol.

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Year:  1993        PMID： 8464356     DOI： 10.1007/bf02536650

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  25 in total

1.  Reaction of glutathione with conjugated carbonyls.

Authors:  H Esterbauer; H Zollner; N Scholz
Journal:  Z Naturforsch C Biosci       Date:  1975 Jul-Aug

2.  Role of cardiac glutathione transferase and of the glutathione S-conjugate export system in biotransformation of 4-hydroxynonenal in the heart.

Authors:  T Ishikawa; H Esterbauer; H Sies
Journal:  J Biol Chem       Date:  1986-02-05       Impact factor: 5.157

3.  Determination of alpha-tocopherol in animal feedstuffs using high-performance liquid chromatography with spectrofluorescence detection.

Authors:  C H McMurray; W J Blanchflower
Journal:  J Chromatogr       Date:  1979-09-01

4.  The role of oxygen free radicals in human disease processes.

Authors:  G B Bulkley
Journal:  Surgery       Date:  1983-09       Impact factor: 3.982

5.  Rapid headspace gas chromatography of hexanal as a measure of lipid peroxidation in biological samples.

Authors:  E N Frankel; M L Hu; A L Tappel
Journal:  Lipids       Date:  1989-11       Impact factor: 1.880

6.  Thiobarbituric acid-reactive substances from peroxidized lipids.

Authors:  H Kosugi; T Kojima; K Kikugawa
Journal:  Lipids       Date:  1989-10       Impact factor: 1.880

7.  Reversible inhibition of DNA and protein synthesis by cumene hydroperoxide and 4-hydroxy-nonenal.

Authors:  M Poot; A Verkerk; J F Koster; H Esterbauer; J F Jongkind
Journal:  Mech Ageing Dev       Date:  1988-04       Impact factor: 5.432

8.  4-Hydroxyalk-2-enals are substrates for glutathione transferase.

Authors:  P Alin; U H Danielson; B Mannervik
Journal:  FEBS Lett       Date:  1985-01-07       Impact factor: 4.124

9.  Detection of 4-hydroxynonenal as a product of lipid peroxidation in native Ehrlich ascites tumor cells.

Authors:  P Winkler; W Lindner; H Esterbauer; E Schauenstein; R J Schaur; G A Khoschsorur
Journal:  Biochim Biophys Acta       Date:  1984-12-06

Review 10.  Methods for determination of aldehydic lipid peroxidation products.

Authors:  H Esterbauer; H Zollner
Journal:  Free Radic Biol Med       Date:  1989       Impact factor: 7.376
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  3 in total

1.  Response of urinary lipophilic aldehydes and related carbonyl compounds to factors that stimulate lipid peroxidation in vivo.

Authors:  A S Csallany; S S Kim; D D Gallaher
Journal:  Lipids       Date:  2000-08       Impact factor: 1.880

2.  Method for analysis of 4-hydroxy-2-(E)-nonenal with solid-phase microextraction.

Authors:  Tatsuhiro Uchida; Naohiro Gotoh; Shun Wada
Journal:  Lipids       Date:  2002-06       Impact factor: 1.880

3.  Transient receptor potential ankyrin-1 has a major role in mediating visceral pain in mice.

Authors:  Fiore Cattaruzza; Ian Spreadbury; Marcela Miranda-Morales; Eileen F Grady; Stephen Vanner; Nigel W Bunnett
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2009-10-29       Impact factor: 4.052
  3 in total

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