Literature DB >> 8463383

Detection of Mycobacterium tuberculosis DNA in clinical samples by using a simple lysis method and polymerase chain reaction.

L Folgueira1, R Delgado, E Palenque, A R Noriega.   

Abstract

We have evaluated the polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculous infection. Two simple methods for mycobacterial DNA release have been compared: sonication and lysis with nonionic detergents and proteinase K. The more effective method was the enzymatic technique. By using this protocol with 75 specimens we detected M. tuberculosis DNA in all of the samples, whereas only 48 and 71 samples were positive by acid-fast staining and culture, respectively.

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Year:  1993        PMID： 8463383      PMCID： PMC263610          DOI： 10.1128/jcm.31.4.1019-1021.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria.

Authors:  G P KUBICA; W E DYE; M L COHN; G MIDDLEBROOK
Journal:  Am Rev Respir Dis       Date:  1963-05

Review 2.  Rapid diagnosis of tuberculosis: laboratory techniques applicable in developing countries.

Authors:  T M Daniel
Journal:  Rev Infect Dis       Date:  1989 Mar-Apr

3.  Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

Authors:  T Victor; R du Toit; P D van Helden
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

4.  Use of polymerase chain reaction for rapid diagnosis of tuberculosis.

Authors:  D V Cousins; S D Wilton; B R Francis; B L Gow
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

5.  Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis.

Authors:  K D Eisenach; M D Cave; J H Bates; J T Crawford
Journal:  J Infect Dis       Date:  1990-05       Impact factor: 5.226

6.  Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction.

Authors:  G E Buck; L C O'Hara; J T Summersgill
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

7.  Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis.

Authors:  D Thierry; A Brisson-Noël; V Vincent-Lévy-Frébault; S Nguyen; J L Guesdon; B Gicquel
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

8.  Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation.

Authors:  A Brisson-Noel; C Aznar; C Chureau; S Nguyen; C Pierre; M Bartoli; R Bonete; G Pialoux; B Gicquel; G Garrigue
Journal:  Lancet       Date:  1991-08-10       Impact factor: 79.321

9.  Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction.

Authors:  K D Eisenach; M D Sifford; M D Cave; J H Bates; J T Crawford
Journal:  Am Rev Respir Dis       Date:  1991-11

10.  Techniques of DNA hybridization detect small numbers of mycobacteria with no cross-hybridization with non-mycobacterial respiratory organisms.

Authors:  S A Shoemaker; J H Fisher; C H Scoggin
Journal:  Am Rev Respir Dis       Date:  1985-05
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  27 in total

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Journal:  J Clin Microbiol       Date:  2006-08-23       Impact factor: 5.948

2.  Design and validation of a low density array (Nosochip) for the detection and identification of the main pathogenic bacteria and fungi responsible for nosocomial pneumonia.

Authors:  S Burteau; P Bogaerts; R de Mendonça; L Irenge; C Berhin; J Hiffe; N de San; P Beyne; S Hamels; Y Glupczynski; M Struelens; J-L Gala; J Remacle
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-01       Impact factor: 3.267

3.  Rapid diagnosis of Mycobacterium tuberculosis bacteremia by PCR.

Authors:  L Folgueira; R Delgado; E Palenque; J M Aguado; A R Noriega
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

4.  Comparative evaluation of two commercial assays for direct detection of Mycobacterium tuberculosis in respiratory specimens.

Authors:  F Gamboa; J M Manterola; J Lonca; L Matas; P J Cardona; E Padilla; B Viñado; J Domínguez; A Hernández; V Ausina
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1998-03       Impact factor: 3.267

5.  Specificities and functions of the recA and pps1 intein genes of Mycobacterium tuberculosis and application for diagnosis of tuberculosis.

Authors:  Isabelle Saves; Lee-Ann Lewis; Fabrice Westrelin; Robin Warren; Mamadou Daffé; Jean-Michel Masson
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

6.  Rapid diagnosis of extrapulmonary tuberculosis by ligase chain reaction amplification.

Authors:  F Gamboa; J Dominguez; E Padilla; J M Manterola; E Gazapo; J Lonca; L Matas; A Hernandez; P J Cardona; V Ausina
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

7.  Comparative evaluation of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.

Authors:  C Piersimoni; A Callegaro; D Nista; S Bornigia; F De Conti; G Santini; G De Sio
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

8.  Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories.

Authors:  G T Noordhoek; A H Kolk; G Bjune; D Catty; J W Dale; P E Fine; P Godfrey-Faussett; S N Cho; T Shinnick; S B Svenson
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

9.  Interferon gamma quantification in cerebrospinal fluid compared with PCR for the diagnosis of tuberculous meningitis.

Authors:  Rafael San Juan; Carmen Sánchez-Suárez; María J Rebollo; Dolores Folgueira; Elia Palenque; Blanca Ortuño; Carlos Lumbreras; José M Aguado
Journal:  J Neurol       Date:  2006-06-19       Impact factor: 4.849

10.  Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA.

Authors:  J A Down; M A O'Connell; M S Dey; A H Walters; D R Howard; M C Little; W E Keating; P Zwadyk; P D Haaland; D A McLaurin; G Cole
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948
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