Literature DB >> 8448432

3D 13C-15N-heteronuclear two-spin coherence spectroscopy for polypeptide backbone assignments in 13C-15N-double-labeled proteins.

T Szyperski1, G Wider, J H Bushweller, K Wüthrich.   

Abstract

The pulse sequence of a new constant-time 3D triple-resonance experiment, ct-HA[CAN]HN, is presented. This experiment delineates exclusively scalar connectivities and uses 13C alpha-15N heteronuclear two-spin coherence to overlay the chemical shift evolution periods of the 13C alpha and 15N nuclei, thereby providing the four resonance frequencies of the alpha-proton, the alpha-carbon, the amide nitrogen, and the amide proton of a given amino acid residue in three dimensions. This experiment promises to be a valid alternative to 4D experiments, providing the same information on intraresidue polypeptide backbone connectivities in 13C-15N-double-labeled proteins.

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Year:  1993        PMID： 8448432     DOI： 10.1007/bf00242481

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  9 in total

1.  Protein secondary structure determination by NMR. Application with recombinant human cyclophilin.

Authors:  K Wüthrich; C Spitzfaden; K Memmert; H Widmer; G Wider
Journal:  FEBS Lett       Date:  1991-07-22       Impact factor: 4.124

2.  An efficient 3D NMR technique for correlating the proton and 15N backbone amide resonances with the alpha-carbon of the preceding residue in uniformly 15N/13C enriched proteins.

Authors:  A Bax; M Ikura
Journal:  J Biomol NMR       Date:  1991-05       Impact factor: 2.835

3.  Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance.

Authors:  K Wüthrich; G Wider; G Wagner; W Braun
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

4.  Staphylococcal nuclease: sequential assignments and solution structure.

Authors:  D A Torchia; S W Sparks; A Bax
Journal:  Biochemistry       Date:  1989-06-27       Impact factor: 3.162

5.  NMR determination of residual structure in a urea-denatured protein, the 434-repressor.

Authors:  D Neri; M Billeter; G Wider; K Wüthrich
Journal:  Science       Date:  1992-09-11       Impact factor: 47.728

6.  Structural and functional characterization of the mutant Escherichia coli glutaredoxin (C14----S) and its mixed disulfide with glutathione.

Authors:  J H Bushweller; F Aslund; K Wüthrich; A Holmgren
Journal:  Biochemistry       Date:  1992-09-29       Impact factor: 3.162

7.  Sequence-specific 1H n.m.r. assignments and determination of the three-dimensional structure of reduced Escherichia coli glutaredoxin.

Authors:  P Sodano; T H Xia; J H Bushweller; O Björnberg; A Holmgren; M Billeter; K Wüthrich
Journal:  J Mol Biol       Date:  1991-10-20       Impact factor: 5.469

8.  A new 3D HN(CA)HA experiment for obtaining fingerprint HN-Halpha peaks in 15N- and 13C-labeled proteins.

Authors:  R T Clubb; V Thanabal; G Wagner
Journal:  J Biomol NMR       Date:  1992-03       Impact factor: 2.835

9.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
  9 in total
  30 in total

Review 1.  Radial sampling for fast NMR: Concepts and practices over three decades.

Authors:  Brian E Coggins; Ronald A Venters; Pei Zhou
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2010-07-30       Impact factor: 9.795

2.  Reduced-dimensionality NMR spectroscopy for high-throughput protein resonance assignment.

Authors:  Thomas Szyperski; Deok C Yeh; Dinesh K Sukumaran; Hunter N B Moseley; Gaetano T Montelione
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

3.  Multiple quadrature detection in reduced dimensionality experiments.

Authors:  Wiktor Koźmiński; Igor Zhukov
Journal:  J Biomol NMR       Date:  2003-06       Impact factor: 2.835

4.  GFT NMR experiments for polypeptide backbone and 13Cbeta chemical shift assignment.

Authors:  Seho Kim; Thomas Szyperski
Journal:  J Biomol NMR       Date:  2004-02       Impact factor: 2.835

5.  Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment.

Authors:  Bernhard Brutscher
Journal:  J Biomol NMR       Date:  2004-05       Impact factor: 2.835

6.  High-resolution iterative frequency identification for NMR as a general strategy for multidimensional data collection.

Authors:  Hamid R Eghbalnia; Arash Bahrami; Marco Tonelli; Klaas Hallenga; John L Markley
Journal:  J Am Chem Soc       Date:  2005-09-14       Impact factor: 15.419

7.  Unambiguous assignment of NMR protein backbone signals with a time-shared triple-resonance experiment.

Authors:  Dominique P Frueh; Haribabu Arthanari; Gerhard Wagner
Journal:  J Biomol NMR       Date:  2005-11       Impact factor: 2.835

8.  Fast acquisition of NMR spectra using Fourier transform of non-equispaced data.

Authors:  Dominique Marion
Journal:  J Biomol NMR       Date:  2005-06       Impact factor: 2.835

9.  PR-CALC: a program for the reconstruction of NMR spectra from projections.

Authors:  Brian E Coggins; Pei Zhou
Journal:  J Biomol NMR       Date:  2006-03       Impact factor: 2.835

10.  High resolution 4-D spectroscopy with sparse concentric shell sampling and FFT-CLEAN.

Authors:  Brian E Coggins; Pei Zhou
Journal:  J Biomol NMR       Date:  2008-10-14       Impact factor: 2.835
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