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Literature DB >> 8434020

In vitro selection of optimal DNA substrates for T4 RNA ligase.

Abstract

We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 RNA ligase. We find that the ensemble of selected sequences ligated about 10 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly, the majority of the selected sequences approximated a well-defined consensus sequence.

Keywords: NASA Discipline Exobiology; NASA Discipline Number 52-20; NASA Program Exobiology; Non-NASA Center

Mesh：

Substances：

Year: 1993 PMID： 8434020 PMCID： PMC45917 DOI： 10.1073/pnas.90.4.1576

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

12 in total

1. In vitro selection of RNAs that undergo autolytic cleavage with Pb2+.

Authors: T Pan; O C Uhlenbeck
Journal: Biochemistry Date: 1992-04-28 Impact factor: 3.162

Review 2. In vitro genetics.

Authors: J W Szostak
Journal: Trends Biochem Sci Date: 1992-03 Impact factor: 13.807

3. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures.

Authors: A D Ellington; J W Szostak
Journal: Nature Date: 1992-02-27 Impact factor: 49.962

4. Directed evolution of an RNA enzyme.

Authors: A A Beaudry; G F Joyce
Journal: Science Date: 1992-07-31 Impact factor: 47.728

5. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.

Authors: C Tuerk; L Gold
Journal: Science Date: 1990-08-03 Impact factor: 47.728

6. In vitro selection of RNA molecules that bind specific ligands.

Authors: A D Ellington; J W Szostak
Journal: Nature Date: 1990-08-30 Impact factor: 49.962

7. Selection of single-stranded DNA molecules that bind and inhibit human thrombin.

Authors: L C Bock; L C Griffin; J A Latham; E H Vermaas; J J Toole
Journal: Nature Date: 1992-02-06 Impact factor: 49.962

Review 8. DNA-joining enzymes: a review.

Authors: N P Higgins; N R Cozzarelli
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

In vitro selection of optimal DNA substrates for T4 RNA ligase.

1. In vitro selection of RNAs that undergo autolytic cleavage with Pb2+.

Review 2. In vitro genetics.

3. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures.

4. Directed evolution of an RNA enzyme.

5. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.

6. In vitro selection of RNA molecules that bind specific ligands.

7. Selection of single-stranded DNA molecules that bind and inhibit human thrombin.

Review 8. DNA-joining enzymes: a review.

9. Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.

10. Bacteriophage T4 anticodon nuclease, polynucleotide kinase and RNA ligase reprocess the host lysine tRNA.

1. A code for transcription initiation in mammalian genomes.

2. Emergence of a dual-catalytic RNA with metal-specific cleavage and ligase activities: the spandrels of RNA evolution.

3. Replication errors during in vivo Ty1 transposition are linked to heterogeneous RNase H cleavage sites.

4. In vitro selection of optimal DNA substrates for ligation by a water-soluble carbodiimide.

5. A primer-free method that selects high-affinity single-stranded DNA aptamers using thermostable RNA ligase.

6. Reducing ligation bias of small RNAs in libraries for next generation sequencing.