Literature DB >> 8432828

Typing of Clostridium difficile by western immunoblotting with 10 different antisera.

H Kato1, J J Cavallaro, N Kato, S L Bartley, G E Killgore, K Watanabe, K Ueno.   

Abstract

Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were tested. Variations in band profiles in each serogroup were used to type isolates into subserogroups. This technique was useful for an epidemiological investigation.

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Year:  1993        PMID: 8432828      PMCID: PMC262776          DOI: 10.1128/jcm.31.2.413-415.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  11 in total

1.  Nosocomial Clostridium difficile colonisation and disease.

Authors:  S Johnson; C R Clabots; F V Linn; M M Olson; L R Peterson; D N Gerding
Journal:  Lancet       Date:  1990-07-14       Impact factor: 79.321

2.  Detection of specific antigens for ten serogroups of Clostridium difficile.

Authors:  M Delmée; V Avesani; I Ernest; M Surleraux
Journal:  Mol Cell Probes       Date:  1990-02       Impact factor: 2.365

3.  Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile.

Authors:  M Delmée; Y Laroche; V Avesani; G Cornelis
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Characterization of a nosocomial Clostridium difficile outbreak by using plasmid profile typing and clindamycin susceptibility testing.

Authors:  C R Clabots; L R Peterson; D N Gerding
Journal:  J Infect Dis       Date:  1988-10       Impact factor: 5.226

6.  Polyacrylamide gel electrophoresis patterns produced by Clostridium difficile.

Authors:  H Wexler; M E Mulligan; S M Finegold
Journal:  Rev Infect Dis       Date:  1984 Mar-Apr

7.  Immunochemical fingerprinting of Clostridium difficile strains isolated from an outbreak of antibiotic-associated colitis and diarrhoea.

Authors:  I R Poxton; B Aronsson; R Möllby; C E Nord; J G Collee
Journal:  J Med Microbiol       Date:  1984-06       Impact factor: 2.472

8.  Serogrouping of Clostridium difficile strains by slide agglutination.

Authors:  M Delmee; M Homel; G Wauters
Journal:  J Clin Microbiol       Date:  1985-03       Impact factor: 5.948

9.  Serotyping of Clostridium difficile.

Authors:  S Toma; G Lesiak; M Magus; H L Lo; M Delmée
Journal:  J Clin Microbiol       Date:  1988-03       Impact factor: 5.948

10.  Acquisition of Clostridium difficile from the hospital environment.

Authors:  G W Kaatz; S D Gitlin; D R Schaberg; K H Wilson; C A Kauffman; S M Seo; R Fekety
Journal:  Am J Epidemiol       Date:  1988-06       Impact factor: 4.897

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  9 in total

1.  Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing.

Authors:  G E Killgore; H Kato
Journal:  J Clin Microbiol       Date:  1994-06       Impact factor: 5.948

2.  Analysis of Clostridium difficile isolates from nosocomial outbreaks at three hospitals in diverse areas of Japan.

Authors:  H Kato; N Kato; K Watanabe; T Yamamoto; K Suzuki; S Ishigo; S Kunihiro; I Nakamura; G E Killgore; S Nakamura
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

3.  Investigation of an outbreak of Clostridium difficile infection in a general hospital by numerical analysis of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  M Costas; B Holmes; S L On; M Ganner; M C Kelly; S K Nath
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

4.  Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping.

Authors:  M Delmée; C Depitre; G Corthier; A Ahoyo; V Avesani
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

5.  Relapses or reinfections: analysis of a case of Clostridium difficile-associated colitis by two typing systems.

Authors:  H Kato; N Kato; K Watanabe; K Ueno; Y Sakata; K Fujita
Journal:  Curr Microbiol       Date:  1996-10       Impact factor: 2.188

6.  Comparison of seven techniques for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, amplified fragment length polymorphism, and surface layer protein A gene sequence typing.

Authors:  George Killgore; Angela Thompson; Stuart Johnson; Jon Brazier; Ed Kuijper; Jacques Pepin; Eric H Frost; Paul Savelkoul; Brad Nicholson; Renate J van den Berg; Haru Kato; Susan P Sambol; Walter Zukowski; Christopher Woods; Brandi Limbago; Dale N Gerding; L Clifford McDonald
Journal:  J Clin Microbiol       Date:  2007-11-26       Impact factor: 5.948

7.  Application of typing by pulsed-field gel electrophoresis to the study of Clostridium difficile in a neonatal intensive care unit.

Authors:  H Kato; N Kato; K Watanabe; K Ueno; H Ushijima; S Hashira; T Abe
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948

8.  Identification of outbreak-associated and other strains of Clostridium difficile by numerical analysis of SDS-PAGE protein patterns.

Authors:  M Costas; B Holmes; M Ganner; S L On; P N Hoffman; M A Worsley; H Panigrahi
Journal:  Epidemiol Infect       Date:  1994-08       Impact factor: 2.451

9.  Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR.

Authors:  H Kato; N Kato; K Watanabe; N Iwai; H Nakamura; T Yamamoto; K Suzuki; S M Kim; Y Chong; E B Wasito
Journal:  J Clin Microbiol       Date:  1998-08       Impact factor: 5.948

  9 in total

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