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Literature DB >> 8408581

Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping.

M Delmée¹, C Depitre, G Corthier, A Ahoyo, V Avesani.

Abstract

An enzyme-linked immunoassay (ELISA) with 11 Clostridium difficile serogroup-specific antisera was applied for serogrouping of C. difficile colonies from 314 consecutive positive fecal samples. Two hundred forty-nine strains (79%) were correctly serogrouped, 57 (18%) belonged to serogroups other than the 11 which were evaluated and gave a negative reaction with all antisera, and 8 isolates (2.5%) did not react with their corresponding antisera. ELISA is a rapid and reliable method for serogrouping C. difficile and should allow for the automation of this procedure.

Entities: Species

Mesh：

Year: 1993 PMID： 8408581 PMCID： PMC265799 DOI： 10.1128/jcm.31.9.2526-2528.1993

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

13 in total

1. Application of whole-cell DNA restriction endonuclease profiles to the epidemiology of Clostridium difficile-induced diarrhea.

Authors: E J Kuijper; J H Oudbier; W N Stuifbergen; A Jansz; H C Zanen
Journal: J Clin Microbiol Date: 1987-04 Impact factor: 5.948

2. Immunoblots and plasmid fingerprints compared with serotyping and polyacrylamide gel electrophoresis for typing Clostridium difficile.

Authors: M E Mulligan; L R Peterson; R Y Kwok; C R Clabots; D N Gerding
Journal: J Clin Microbiol Date: 1988-01 Impact factor: 5.948

3. Bacterial agglutination and polyacrylamide gel electrophoresis for typing Clostridium difficile.

Authors: M E Mulligan; S Halebian; R Y Kwok; W C Cheng; S M Finegold; C R Anselmo; D N Gerding; L R Peterson
Journal: J Infect Dis Date: 1986-02 Impact factor: 5.226

4. Characterization of flagella of Clostridium difficile and their role in serogrouping reactions.

Authors: M Delmée; V Avesani; N Delferriere; G Burtonboy
Journal: J Clin Microbiol Date: 1990-10 Impact factor: 5.948

5. Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies.

Authors: S Tabaqchali; D Holland; S O'Farrell; R Silman
Journal: Lancet Date: 1984-04-28 Impact factor: 79.321

6. Application of a technique for serogrouping Clostridium difficile in an outbreak of antibiotic-associated diarrhoea.

Authors: M Delmee; G Bulliard; G Simon
Journal: J Infect Date: 1986-07 Impact factor: 6.072

7. Typing of Clostridium difficile by western immunoblotting with 10 different antisera.

Authors: H Kato; J J Cavallaro; N Kato; S L Bartley; G E Killgore; K Watanabe; K Ueno
Journal: J Clin Microbiol Date: 1993-02 Impact factor: 5.948

8. Selective and differential medium for isolation of Clostridium difficile.

Authors: W L George; V L Sutter; D Citron; S M Finegold
Journal: J Clin Microbiol Date: 1979-02 Impact factor: 5.948

9. Serogrouping of Clostridium difficile strains by slide agglutination.

Authors: M Delmee; M Homel; G Wauters
Journal: J Clin Microbiol Date: 1985-03 Impact factor: 5.948

10. Serotyping of Clostridium difficile.

Authors: S Toma; G Lesiak; M Magus; H L Lo; M Delmée
Journal: J Clin Microbiol Date: 1988-03 Impact factor: 5.948

7 in total

1. Epidemiology of recurrences or reinfections of Clostridium difficile-associated diarrhea.

Authors: F Barbut; A Richard; K Hamadi; V Chomette; B Burghoffer; J C Petit
Journal: J Clin Microbiol Date: 2000-06 Impact factor: 5.948

2. Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

Authors: P Bidet; V Lalande; B Salauze; B Burghoffer; V Avesani; M Delmée; A Rossier; F Barbut; J C Petit
Journal: J Clin Microbiol Date: 2000-07 Impact factor: 5.948

3. Antimicrobial susceptibilities and serogroups of clinical strains of Clostridium difficile isolated in France in 1991 and 1997.

Authors: F Barbut; D Decré; B Burghoffer; D Lesage; F Delisle; V Lalande; M Delmée; V Avesani; N Sano; C Coudert; J C Petit
Journal: Antimicrob Agents Chemother Date: 1999-11 Impact factor: 5.191

4. Prevalence and genetic characterization of toxin A variant strains of Clostridium difficile among adults and children with diarrhea in France.

Authors: Frédéric Barbut; Valérie Lalande; Béatrice Burghoffer; Huong Vu Thien; Emmanuel Grimprel; Jean-Claude Petit
Journal: J Clin Microbiol Date: 2002-06 Impact factor: 5.948

5. gyrA and gyrB mutations are implicated in cross-resistance to Ciprofloxacin and moxifloxacin in Clostridium difficile.

Authors: Larbi Dridi; Jacques Tankovic; Béatrice Burghoffer; Frédéric Barbut; Jean-Claude Petit
Journal: Antimicrob Agents Chemother Date: 2002-11 Impact factor: 5.191

6. Nosocomial outbreak of Clostridium difficile diarrhea in a pediatric service.

Authors: A Ferroni; J Merckx; T Ancelle; B Pron; E Abachin; F Barbut; J Larzul; P Rigault; P Berche; J L Gaillard
Journal: Eur J Clin Microbiol Infect Dis Date: 1997-12 Impact factor: 3.267

7. Prevalence and characterization of a binary toxin (actin-specific ADP-ribosyltransferase) from Clostridium difficile.

Authors: Carina Gonçalves; Dominique Decré; Frédéric Barbut; Béatrice Burghoffer; Jean-Claude Petit
Journal: J Clin Microbiol Date: 2004-05 Impact factor: 5.948

7 in total