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Literature DB >> 3782463

Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile.

M Delmée, Y Laroche, V Avesani, G Cornelis.

Abstract

A typing scheme for Clostridium difficile based on slide agglutination with rabbit antisera was previously described. It allows the differentiation of 10 serogroups designated A, B, C, D, F, G, H, I, K, and X. We studied the correlation between serogrouping and polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins. A total of 202 isolates from different sources were analyzed by PAGE after ultrasonic disintegration of cells from an 18-h liquid culture and treatment with sodium dodecyl sulfate and 2-mercaptoethanol. A total of 21 different patterns were observed. The reference strains from the 10 serogroups showed different profiles. For each serogroup except A, the patterns obtained with the clinical isolates were identical to the patterns obtained with the reference strains. For the 48 strains belonging to serogroup A, 12 different profiles were observed. Five of these involved strains isolated from patients with antibiotic-associated diarrhea. Typing by sodium dodecyl sulfate-PAGE thus correlates with serogrouping. In addition, it allows discrimination within the heterogeneous serogroup A.

Entities: Chemical Disease Species

Mesh：

Substances：
Bacterial Proteins

Year: 1986 PMID： 3782463 PMCID： PMC269085 DOI： 10.1128/jcm.24.6.991-994.1986

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

22 in total

1. Pseudomembranous colitis in a 5-week-old infant.

Authors: B K Mandal; B Watson; M Ellis
Journal: Br Med J (Clin Res Ed) Date: 1982-01-30

2. Is Clostridium difficile pathogenic in infants?

Authors: D F Welch; M I Marks
Journal: J Pediatr Date: 1982-03 Impact factor: 4.406

3. Pseudomembranous colitis in a leukaemia unit: a report of five fatal cases.

Authors: D W Milligan; J K Kelly
Journal: J Clin Pathol Date: 1979-12 Impact factor: 3.411

4. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia.

Authors: J G Bartlett; T W Chang; M Gurwith; S L Gorbach; A B Onderdonk
Journal: N Engl J Med Date: 1978-03-09 Impact factor: 91.245

5. The prevalence of Clostridium difficile and toxin in a nursery population: a comparison between patients with necrotizing enterocolitis and an asymptomatic group.

Authors: R J Sherertz; F A Sarubbi
Journal: J Pediatr Date: 1982-03 Impact factor: 4.406

6. Epidemiologic study of necrotizing enterocolitis among low-birth-weight infants. Absence of identifiable risk factors.

Authors: R M Kliegman; M Hack; P Jones; A A Fanaroff
Journal: J Pediatr Date: 1982-03 Impact factor: 4.406

7. Investigation of an outbreak of antibiotic-associated colitis by various typing methods.

Authors: J Wüst; N M Sullivan; U Hardegger; T D Wilkins
Journal: J Clin Microbiol Date: 1982-12 Impact factor: 5.948

8. Application of a technique for serogrouping Clostridium difficile in an outbreak of antibiotic-associated diarrhoea.

Authors: M Delmee; G Bulliard; G Simon
Journal: J Infect Date: 1986-07 Impact factor: 6.072

9. Epidemiology of Clostridium difficile in infants.

Authors: H E Larson; F E Barclay; P Honour; I D Hill
Journal: J Infect Dis Date: 1982-12 Impact factor: 5.226

10. Aetiology of antimicrobial-agent-associated colitis.

Authors: W L George; V L Sutter; E J Goldstein; S L Ludwig; S M Finegold
Journal: Lancet Date: 1978-04-15 Impact factor: 79.321

33 in total

1. Molecular characterization of fliD gene encoding flagellar cap and its expression among Clostridium difficile isolates from different serogroups.

Authors: A Tasteyre; T Karjalainen; V Avesani; M Delmée; A Collignon; P Bourlioux; M C Barc
Journal: J Clin Microbiol Date: 2001-03 Impact factor: 5.948

2. Phenotypic and genotypic diversity of the flagellin gene (fliC) among Clostridium difficile isolates from different serogroups.

Authors: A Tasteyre; T Karjalainen; V Avesani; M Delmée; A Collignon; P Bourlioux; M C Barc
Journal: J Clin Microbiol Date: 2000-09 Impact factor: 5.948

3. Restriction endonuclease analysis of nosocomial isolates of Clostridium difficile.

Authors: H R Devlin; W Au; L Foux; W C Bradbury
Journal: J Clin Microbiol Date: 1987-11 Impact factor: 5.948

4. Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B.

Authors: A C Fluit; M J Wolfhagen; G P Verdonk; M Jansze; R Torensma; J Verhoef
Journal: J Clin Microbiol Date: 1991-11 Impact factor: 5.948

5. Immunoblots and plasmid fingerprints compared with serotyping and polyacrylamide gel electrophoresis for typing Clostridium difficile.

Authors: M E Mulligan; L R Peterson; R Y Kwok; C R Clabots; D N Gerding
Journal: J Clin Microbiol Date: 1988-01 Impact factor: 5.948

6. Human antibody response to Clostridium difficile toxin A in relation to clinical course of infection.

Authors: M Warny; J P Vaerman; V Avesani; M Delmée
Journal: Infect Immun Date: 1994-02 Impact factor: 3.441

7. Investigation of an outbreak of Clostridium difficile infection in a general hospital by numerical analysis of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: M Costas; B Holmes; S L On; M Ganner; M C Kelly; S K Nath
Journal: J Clin Microbiol Date: 1994-03 Impact factor: 5.948