Literature DB >> 8415019

Addition of a class IIS enzyme site in the mutagenic primer to improve two-step PCR-based targeted mutagenesis.

J Armengaud1, Y Jouanneau.   

Abstract

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Year:  1993        PMID： 8415019      PMCID： PMC310102          DOI： 10.1093/nar/21.18.4424

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  6 in total

1.  Improved primer design for PCR-based, site-directed mutagenesis.

Authors:  A D Sharrocks; P E Shaw
Journal:  Nucleic Acids Res       Date:  1992-03-11       Impact factor: 16.971

2.  A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

Authors:  O Landt; H P Grunert; U Hahn
Journal:  Gene       Date:  1990-11-30       Impact factor: 3.688

3.  Improved site-directed mutagenesis method using PCR.

Authors:  O P Kuipers; H J Boot; W M de Vos
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

4.  Nucleotide sequence of fdxA encoding a 7Fe ferredoxin of Rhodobacter capsulatus.

Authors:  C Duport; Y Jouanneau; P M Vignais
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

5.  Using the polymerase chain reaction to modify expression plasmids for epitope mapping.

Authors:  S E Mole; R D Iggo; D P Lane
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

6.  Ksp632I, a novel class-IIS restriction endonuclease from Kluyvera sp. strain 632 with the asymmetric hexanucleotide recognition sequence: 5'-CTCTTC(N)1-3' 3'-GAGAAG(N)4-5'.

Authors:  B J Bolton; G G Schmitz; M Jarsch; M J Comer; C Kessler
Journal:  Gene       Date:  1988-06-15       Impact factor: 3.688
  6 in total
  1 in total

1.  An efficient PCR mutagenesis strategy without gel purification [correction of "purificiation"] step that is amenable to automation.

Authors:  B Séraphin; S Kandels-Lewis
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971
  1 in total

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