Literature DB >> 1886781

Improved site-directed mutagenesis method using PCR.

O P Kuipers1, H J Boot, W M de Vos.   

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Year:  1991        PMID: 1886781      PMCID: PMC328655          DOI: 10.1093/nar/19.16.4558

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


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  4 in total

1.  A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

Authors:  O Landt; H P Grunert; U Hahn
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2.  Using the polymerase chain reaction to modify expression plasmids for epitope mapping.

Authors:  S E Mole; R D Iggo; D P Lane
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

3.  Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.

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4.  Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.

Authors:  G W Buchman; S Banerjee; J N Hansen
Journal:  J Biol Chem       Date:  1988-11-05       Impact factor: 5.157

  4 in total
  41 in total

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2.  The design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysis.

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3.  A general and fast method to generate multiple site directed mutations.

Authors:  I Mikaelian; A Sergeant
Journal:  Nucleic Acids Res       Date:  1992-01-25       Impact factor: 16.971

4.  Improved primer design for PCR-based, site-directed mutagenesis.

Authors:  A D Sharrocks; P E Shaw
Journal:  Nucleic Acids Res       Date:  1992-03-11       Impact factor: 16.971

5.  Analysis of novel soluble chromate and uranyl reductases and generation of an improved enzyme by directed evolution.

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6.  Genes involved in control of galactose uptake in Lactobacillus brevis and reconstitution of the regulatory system in Bacillus subtilis.

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7.  Distribution and evolution of nisin-sucrose elements in Lactococcus lactis.

Authors:  P J Rauch; M M Beerthuyzen; W M de Vos
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8.  The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast.

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9.  Disease-causing mutations in exon 11 of the medium-chain acyl-CoA dehydrogenase gene.

Authors:  B S Andresen; T G Jensen; P Bross; I Knudsen; V Winter; S Kølvraa; L Bolund; J H Ding; Y T Chen; J L Van Hove
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10.  Negatively charged amino acid residues in the nicotinic receptor delta subunit that contribute to the binding of acetylcholine.

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