Literature DB >> 1549485

Improved primer design for PCR-based, site-directed mutagenesis.

A D Sharrocks1, P E Shaw.   

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Year:  1992        PMID： 1549485      PMCID： PMC312111          DOI： 10.1093/nar/20.5.1147

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  4 in total

1.  A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

Authors:  O Landt; H P Grunert; U Hahn
Journal:  Gene       Date:  1990-11-30       Impact factor: 3.688

2.  Improved site-directed mutagenesis method using PCR.

Authors:  O P Kuipers; H J Boot; W M de Vos
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

3.  Using the polymerase chain reaction to modify expression plasmids for epitope mapping.

Authors:  S E Mole; R D Iggo; D P Lane
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

4.  Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.

Authors:  C Norman; M Runswick; R Pollock; R Treisman
Journal:  Cell       Date:  1988-12-23       Impact factor: 41.582
  4 in total
  14 in total

1.  Furin is involved in baculovirus envelope fusion protein activation.

Authors:  Marcel Westenberg; Hualin Wang; Wilfred F J IJkel; Rob W Goldbach; Just M Vlak; Douwe Zuidema
Journal:  J Virol       Date:  2002-01       Impact factor: 5.103

2.  Functional analysis of the putative fusion domain of the baculovirus envelope fusion protein F.

Authors:  Marcel Westenberg; Frank Veenman; Els C Roode; Rob W Goldbach; Just M Vlak; Douwe Zuidema
Journal:  J Virol       Date:  2004-07       Impact factor: 5.103

3.  PrimerCE: designing primers for cloning and gene expression.

Authors:  Yinghao Cao; Jian Sun; Jianhui Zhu; Liyun Li; Guozhen Liu
Journal:  Mol Biotechnol       Date:  2010-10       Impact factor: 2.695

4.  Determinants of DNA-binding specificity of ETS-domain transcription factors.

Authors:  P Shore; A J Whitmarsh; R Bhaskaran; R J Davis; J P Waltho; A D Sharrocks
Journal:  Mol Cell Biol       Date:  1996-07       Impact factor: 4.272

5.  An efficient PCR mutagenesis strategy without gel purification [correction of "purificiation"] step that is amenable to automation.

Authors:  B Séraphin; S Kandels-Lewis
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

6.  Identification of amino acids essential for DNA binding and dimerization in p67SRF: implications for a novel DNA-binding motif.

Authors:  A D Sharrocks; H Gille; P E Shaw
Journal:  Mol Cell Biol       Date:  1993-01       Impact factor: 4.272

7.  Addition of a class IIS enzyme site in the mutagenic primer to improve two-step PCR-based targeted mutagenesis.

Authors:  J Armengaud; Y Jouanneau
Journal:  Nucleic Acids Res       Date:  1993-09-11       Impact factor: 16.971

8.  Intronic and flanking sequences are required to silence enhancement of an embryonic beta-type globin gene.

Authors:  N J Wandersee; R C Ferris; G D Ginder
Journal:  Mol Cell Biol       Date:  1996-01       Impact factor: 4.272

9.  The identification of elements determining the different DNA binding specificities of the MADS box proteins p67SRF and RSRFC4.

Authors:  A D Sharrocks; F von Hesler; P E Shaw
Journal:  Nucleic Acids Res       Date:  1993-01-25       Impact factor: 16.971

10.  The transcription factors Elk-1 and serum response factor interact by direct protein-protein contacts mediated by a short region of Elk-1.

Authors:  P Shore; A D Sharrocks
Journal:  Mol Cell Biol       Date:  1994-05       Impact factor: 4.272
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