Literature DB >> 8408541

Construction of a bioluminescent mycobacterium and its use for assay of antimycobacterial agents.

P W Andrew1, I S Roberts.   

Abstract

To show, as a model system, that mycobacteria can express heterologous luciferase genes and that bioluminescence can be a rapid method of measuring antimycobacterial activity, a bioluminescent form of Mycobacterium smegmatis was made by transformation with a Mycobacterium-Escherichia coli shuttle vector containing the luxAB genes from Vibrio harveyi. The antimycobacterial effects of antibiotics and biocides could be assayed in real time by using bioluminescent M. smegmatis.

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Year:  1993        PMID： 8408541      PMCID： PMC265743          DOI： 10.1128/jcm.31.9.2251-2254.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

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