Literature DB >> 8393824

M13 and pUC vectors with new unique restriction sites for cloning.

V Benes1, Z Hostomský, L Arnold, V Paces.   

Abstract

Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessential part of the vector molecule was changed into a BssHII site, so that the NarI site in the MCS became unique.

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Year:  1993        PMID： 8393824     DOI： 10.1016/0378-1119(93)90360-f

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  19 in total

1.  Fusions between green fluorescent protein and beta-glucuronidase as sensitive and vital bifunctional reporters in plants.

Authors:  N E Quaedvlieg; H R Schlaman; P C Admiraal; S E Wijting; J Stougaard; H P Spaink
Journal:  Plant Mol Biol       Date:  1998-11       Impact factor: 4.076

2.  APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae.

Authors:  J D Alfonzo; T R Crother; M L Guetsova; B Daignan-Fornier; M W Taylor
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

3.  Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri.

Authors:  N C Heng; H F Jenkinson; G W Tannock
Journal:  Appl Environ Microbiol       Date:  1997-08       Impact factor: 4.792

4.  HbpR, a new member of the XylR/DmpR subclass within the NtrC family of bacterial transcriptional activators, regulates expression of 2-hydroxybiphenyl metabolism in Pseudomonas azelaica HBP1.

Authors:  M C Jaspers; W A Suske; A Schmid; D A Goslings; H P Kohler; J R van der Meer
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

5.  Identification of Lactobacillus reuteri genes specifically induced in the mouse gastrointestinal tract.

Authors:  Jens Walter; Nicholas C K Heng; Walter P Hammes; Diane M Loach; Gerald W Tannock; Christian Hertel
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6.  Influence of different functional elements of plasmid pGT232 on maintenance of recombinant plasmids in Lactobacillus reuteri populations in vitro and in vivo.

Authors:  N C Heng; J M Bateup; D M Loach; X Wu; H F Jenkinson; M Morrison; G W Tannock
Journal:  Appl Environ Microbiol       Date:  1999-12       Impact factor: 4.792

7.  Threonine-11, phosphorylated by Rad3 and atm in vitro, is required for activation of fission yeast checkpoint kinase Cds1.

Authors:  K Tanaka; M N Boddy; X B Chen; C H McGowan; P Russell
Journal:  Mol Cell Biol       Date:  2001-05       Impact factor: 4.272

8.  Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus.

Authors:  Patrizia Contursi; Francesca M Pisani; Andrei Grigoriev; Raffaele Cannio; Simonetta Bartolucci; Mosè Rossi
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9.  Int-B13, an unusual site-specific recombinase of the bacteriophage P4 integrase family, is responsible for chromosomal insertion of the 105-kilobase clc element of Pseudomonas sp. Strain B13.

Authors:  R Ravatn; S Studer; A J Zehnder; J R van der Meer
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

10.  Fusions between green fluorescent protein and beta-glucuronidase as sensitive and vital bifunctional reporters in plants.

Authors:  N E Quaedvlieg; H R Schlaman; P C Admiraal; S E Wijting; J Stougaard; H P Spaink
Journal:  Plant Mol Biol       Date:  1998-07       Impact factor: 4.076
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