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Literature DB >> 9251227

Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri.

Abstract

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.

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Year: 1997 PMID： 9251227 PMCID： PMC168638 DOI： 10.1128/aem.63.8.3336-3340.1997

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

15 in total

1. High efficiency transformation of E. coli by high voltage electroporation.

Authors: W J Dower; J F Miller; C W Ragsdale
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2. Lactobacillus succession in the piglet digestive tract demonstrated by plasmid profiling.

Authors: G W Tannock; R Fuller; K Pedersen
Journal: Appl Environ Microbiol Date: 1990-05 Impact factor: 4.792

3. Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.

Authors: E E Bates; H J Gilbert; G P Hazlewood; J Huckle; J I Laurie; S P Mann
Journal: Appl Environ Microbiol Date: 1989-08 Impact factor: 4.792

4. Structural genes encoding the thermophilic alpha-amylases of Bacillus stearothermophilus and Bacillus licheniformis.

Authors: G L Gray; S E Mainzer; M W Rey; M H Lamsa; K L Kindle; C Carmona; C Requadt
Journal: J Bacteriol Date: 1986-05 Impact factor: 3.490

5. Hybrid Bacillus (1-3,1-4)-beta-glucanases: engineering thermostable enzymes by construction of hybrid genes.

Authors: O Olsen; R Borriss; O Simon; K K Thomsen
Journal: Mol Gen Genet Date: 1991-02

6. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.

Authors: S G Grant; J Jessee; F R Bloom; D Hanahan
Journal: Proc Natl Acad Sci U S A Date: 1990-06 Impact factor: 11.205

7. Structure of the beta-1,3-1,4-glucanase gene of Bacillus macerans: homologies to other beta-glucanases.

Authors: R Borriss; K Buettner; P Maentsaelae
Journal: Mol Gen Genet Date: 1990-07

8. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.

Authors: R M Teather; P J Wood
Journal: Appl Environ Microbiol Date: 1982-04 Impact factor: 4.792

9. Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-beta-glucanase.

Authors: M Hahn; O Olsen; O Politz; R Borriss; U Heinemann
Journal: J Biol Chem Date: 1995-02-17 Impact factor: 5.157

10. Assay of reducing sugars in the nanomole range with 2,2'-bicinchoninate.

Authors: S Waffenschmidt; L Jaenicke
Journal: Anal Biochem Date: 1987-09 Impact factor: 3.365

6 in total

Review 1. A special fondness for lactobacilli.

Authors: Gerald W Tannock
Journal: Appl Environ Microbiol Date: 2004-06 Impact factor: 4.792