| Literature DB >> 8378302 |
Abstract
It is difficult to identify sex in many species of eukaryotic organism. This can considerably impede research into their biology. Fortunately, one sex often possesses a unique chromosome termed Y or W. When DNA markers are available for these chromosomes, then sex identification becomes straightforward. We describe a technique that facilitates the isolation of such markers. The procedure makes use of low-stringency PCR to screen randomly selected primers for their ability to amplify sex-specific loci.Mesh:
Substances:
Year: 1993 PMID: 8378302 PMCID: PMC47348 DOI: 10.1073/pnas.90.18.8324
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205