Literature DB >> 8378302

The isolation of molecular genetic markers for the identification of sex.

R Griffiths1, B Tiwari.   

Abstract

It is difficult to identify sex in many species of eukaryotic organism. This can considerably impede research into their biology. Fortunately, one sex often possesses a unique chromosome termed Y or W. When DNA markers are available for these chromosomes, then sex identification becomes straightforward. We describe a technique that facilitates the isolation of such markers. The procedure makes use of low-stringency PCR to screen randomly selected primers for their ability to amplify sex-specific loci.

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Year:  1993        PMID: 8378302      PMCID: PMC47348          DOI: 10.1073/pnas.90.18.8324

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  10 in total

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Authors:  P D Griffiths
Journal:  Infect Agents Dis       Date:  1992-10

2.  A simple method for subcloning DNA fragments from gel slices.

Authors:  D M Heery; F Gannon; R Powell
Journal:  Trends Genet       Date:  1990-06       Impact factor: 11.639

3.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

4.  Biopsy of human preimplantation embryos and sexing by DNA amplification.

Authors:  A H Handyside; J K Pattinson; R J Penketh; J D Delhanty; R M Winston; E G Tuddenham
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5.  Fingerprinting genomes using PCR with arbitrary primers.

Authors:  J Welsh; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

6.  A novel avian W chromosome DNA repeat sequence in the lesser black-backed gull (Larus fuscus).

Authors:  R Griffiths; P W Holland
Journal:  Chromosoma       Date:  1990-08       Impact factor: 4.316

7.  Genus specificity and extensive methylation of the W chromosome-specific repetitive DNA sequences from the domestic fowl, Gallus gallus domesticus.

Authors:  M Tone; Y Sakaki; T Hashiguchi; S Mizuno
Journal:  Chromosoma       Date:  1984       Impact factor: 4.316

8.  The identification of sex in the starling Sturnus vulgaris using a molecular DNA technique.

Authors:  R Griffiths; B Tiwari; S A Becher
Journal:  Mol Ecol       Date:  1992-10       Impact factor: 6.185

9.  A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif.

Authors:  A H Sinclair; P Berta; M S Palmer; J R Hawkins; B L Griffiths; M J Smith; J W Foster; A M Frischauf; R Lovell-Badge; P N Goodfellow
Journal:  Nature       Date:  1990-07-19       Impact factor: 49.962

10.  Evolution of sex determination and the Y chromosome: SRY-related sequences in marsupials.

Authors:  J W Foster; F E Brennan; G K Hampikian; P N Goodfellow; A H Sinclair; R Lovell-Badge; L Selwood; M B Renfree; D W Cooper; J A Graves
Journal:  Nature       Date:  1992-10-08       Impact factor: 49.962

  10 in total
  8 in total

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Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2002-03-29       Impact factor: 6.237

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5.  Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing carinatae birds.

Authors:  A Ogawa; I Solovei; N Hutchison; Y Saitoh; J E Ikeda; H Macgregor; S Mizuno
Journal:  Chromosome Res       Date:  1997-04       Impact factor: 5.239

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Journal:  Proc Natl Acad Sci U S A       Date:  2017-06-12       Impact factor: 11.205

7.  Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes.

Authors:  I Stehlik; F R Blattner
Journal:  Theor Appl Genet       Date:  2003-09-13       Impact factor: 5.699

8.  Eggshell membrane for DNA sexing of the endangered Maleo ( Macrocephalon maleo).

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  8 in total

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