Literature DB >> 8376352

The desA gene of the cyanobacterium Synechocystis sp. strain PCC6803 is the structural gene for delta 12 desaturase.

H Wada1, M H Avelange-Macherel, N Murata.   

Abstract

The desA gene of the cyanobacterium Synechocystis sp. strain PCC6803 was expressed in Escherichia coli, which does not contain any fatty acid desaturase. The product of the desA gene catalyzed the desaturation of fatty acids at the delta 12 position. This result demonstrates that desA is the structural gene for a delta 12 desaturase.

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Year:  1993        PMID： 8376352      PMCID： PMC206691          DOI： 10.1128/jb.175.18.6056-6058.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  7 in total

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Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
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3.  Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation.

Authors:  H Wada; Z Gombos; N Murata
Journal:  Nature       Date:  1990-09-13       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

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6.  In vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes.

Authors:  H Wada; H Schmidt; E Heinz; N Murata
Journal:  J Bacteriol       Date:  1993-01       Impact factor: 3.490

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Authors:  M Esfahani; E M Barnes; S J Wakil
Journal:  Proc Natl Acad Sci U S A       Date:  1969-11       Impact factor: 11.205
  7 in total
  12 in total

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Authors:  N Murata; H Wada
Journal:  Biochem J       Date:  1995-05-15       Impact factor: 3.857

2.  Identification of a gene that complements an Arabidopsis mutant deficient in chloroplast omega 6 desaturase activity.

Authors:  D L Falcone; S Gibson; B Lemieux; C Somerville
Journal:  Plant Physiol       Date:  1994-12       Impact factor: 8.340

3.  The significance of C16 fatty acids in the sn-2 positions of glycerolipids in the photosynthetic growth of Synechocystis sp. PCC6803.

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7.  Immunocytochemical localization of acyl-lipid desaturases in cyanobacterial cells: evidence that both thylakoid membranes and cytoplasmic membranes are sites of lipid desaturation.

Authors:  L Mustardy; D A Los; Z Gombos; N Murata
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

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10.  Bacterial Genome Mining of Enzymatic Tools for Alkyne Biosynthesis.

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