Literature DB >> 27561030

A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool.

Xuejun Zhu1, Peyton Shieh, Michael Su, Carolyn R Bertozzi, Wenjun Zhang.   

Abstract

Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.

Entities:  

Year:  2016        PMID： 27561030      PMCID： PMC5023497          DOI： 10.1039/c6cc05990b

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  28 in total

Review 1.  Chemistry in living systems.

Authors:  Jennifer A Prescher; Carolyn R Bertozzi
Journal:  Nat Chem Biol       Date:  2005-06       Impact factor: 15.040

Review 2.  Design strategies for bioorthogonal smart probes.

Authors:  Peyton Shieh; Carolyn R Bertozzi
Journal:  Org Biomol Chem       Date:  2014-10-15       Impact factor: 3.876

3.  Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase.

Authors:  Alena Busche; Daniel Gottstein; Christopher Hein; Nina Ripin; Irina Pader; Peter Tufar; Eli B Eisman; Liangcai Gu; Christopher T Walsh; David H Sherman; Frank Löhr; Peter Güntert; Volker Dötsch
Journal:  ACS Chem Biol       Date:  2011-12-15       Impact factor: 5.100

4.  In vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes.

Authors:  H Wada; H Schmidt; E Heinz; N Murata
Journal:  J Bacteriol       Date:  1993-01       Impact factor: 3.490

5.  Crystal structure of human stearoyl-coenzyme A desaturase in complex with substrate.

Authors:  Hui Wang; Michael G Klein; Hua Zou; Weston Lane; Gyorgy Snell; Irena Levin; Ke Li; Bi-Ching Sang
Journal:  Nat Struct Mol Biol       Date:  2015-06-22       Impact factor: 15.369

6.  Biosynthetic genes and activity spectrum of antifungal polyynes from Collimonas fungivorans Ter331.

Authors:  Kathrin Fritsche; Marlies van den Berg; Wietse de Boer; Teris A van Beek; Jos M Raaijmakers; Johannes A van Veen; Johan H J Leveau
Journal:  Environ Microbiol       Date:  2014-03-27       Impact factor: 5.491

7.  The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles.

Authors:  Victoria S Haritos; Irene Horne; Katherine Damcevski; Karen Glover; Nerida Gibb; Shoko Okada; Mats Hamberg
Journal:  Nat Commun       Date:  2012       Impact factor: 14.919

8.  Bacterial Genome Mining of Enzymatic Tools for Alkyne Biosynthesis.

Authors:  Xuejun Zhu; Michael Su; Kadhirvel Manickam; Wenjun Zhang
Journal:  ACS Chem Biol       Date:  2015-10-12       Impact factor: 5.100

9.  CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum.

Authors:  Peyton Shieh; Vivian T Dien; Brendan J Beahm; Joseph M Castellano; Tony Wyss-Coray; Carolyn R Bertozzi
Journal:  J Am Chem Soc       Date:  2015-06-02       Impact factor: 15.419

10.  X-ray structure of a mammalian stearoyl-CoA desaturase.

Authors:  Yonghong Bai; Jason G McCoy; Elena J Levin; Pablo Sobrado; Kanagalaghatta R Rajashankar; Brian G Fox; Ming Zhou
Journal:  Nature       Date:  2015-06-22       Impact factor: 49.962
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  2 in total

1.  Nature's Combinatorial Biosynthesis Produces Vatiamides A-F.

Authors:  Nathan A Moss; Grant Seiler; Tiago F Leão; Gabriel Castro-Falcón; Lena Gerwick; Chambers C Hughes; William H Gerwick
Journal:  Angew Chem Int Ed Engl       Date:  2019-05-28       Impact factor: 15.336

2.  Engineered Biosynthesis of Alkyne-Tagged Polyketides by Type I PKSs.

Authors:  William B Porterfield; Nannalin Poenateetai; Wenjun Zhang
Journal:  iScience       Date:  2020-02-26
  2 in total

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