Literature DB >> 8357267

Colony immunoblot assay of botulinal toxin.

M C Goodnough1, B Hammer, H Sugiyama, E A Johnson.   

Abstract

Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8357267      PMCID： PMC182282          DOI： 10.1128/aem.59.7.2339-2342.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Clostridium botulinum types A, B, C1, and E produce proteins with or without hemagglutinating activity: do they share common amino acid sequences and genes?

Authors:  E Somers; B R DasGupta
Journal:  J Protein Chem       Date:  1991-08

2.  Molecular forms of neurotoxins in proteolytic Clostridium botulinum type B cultures.

Authors:  B R Dasgupta; H Sugiyama
Journal:  Infect Immun       Date:  1976-09       Impact factor: 3.441

3.  Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, C1, D, and E toxins and tetanus toxin.

Authors:  K Tsuzuki; N Yokosawa; B Syuto; I Ohishi; N Fujii; K Kimura; K Oguma
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441

4.  Simplified purification method for Clostridium botulinum type E toxin.

Authors:  J A Giménez; H Sugiyama
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

5.  Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A.

Authors:  S Notermans; J Dufrenne; M Schothorst
Journal:  Jpn J Med Sci Biol       Date:  1978-02

6.  Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum.

Authors:  D A Boroff; U Fleck
Journal:  J Bacteriol       Date:  1966-11       Impact factor: 3.490

7.  Selective medium for isolation of Clostridium botulinum from human feces.

Authors:  M Dezfulian; L M McCroskey; C L Hatheway; V R Dowell
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948

8.  Preparation and characterisation of homogeneous neurotoxin type A from Clostridium botulinum. Its inhibitory action on neuronal release of acetylcholine in the absence and presence of beta-bungarotoxin.

Authors:  C K Tse; J O Dolly; P Hambleton; D Wray; J Melling
Journal:  Eur J Biochem       Date:  1982-03-01
  8 in total
  9 in total

1.  Characterization of Clostridium botulinum strains associated with an infant botulism case in the United Kingdom.

Authors:  Eric A Johnson; William H Tepp; Marite Bradshaw; Richard J Gilbert; Paul E Cook; E David G McIntosh
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

2.  Distribution of Clostridium botulinum type E strains in Nunavik, Northern Quebec, Canada.

Authors:  Daniel Leclair; Jeffrey M Farber; Bill Doidge; Burke Blanchfield; Sandy Suppa; Franco Pagotto; John W Austin
Journal:  Appl Environ Microbiol       Date:  2012-11-16       Impact factor: 4.792

3.  Holotoxin Activity of Botulinum Neurotoxin Subtype A4 Originating from a Nontoxigenic Clostridium botulinum Expression System.

Authors:  Marite Bradshaw; William H Tepp; Regina C M Whitemarsh; Sabine Pellett; Eric A Johnson
Journal:  Appl Environ Microbiol       Date:  2014-09-19       Impact factor: 4.792

4.  Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.

Authors:  Regina C M Whitemarsh; Monica J Strathman; Lucas G Chase; Casey Stankewicz; William H Tepp; Eric A Johnson; Sabine Pellett
Journal:  Toxicol Sci       Date:  2012-01-05       Impact factor: 4.849

Review 5.  Laboratory diagnostics of botulism.

Authors:  Miia Lindström; Hannu Korkeala
Journal:  Clin Microbiol Rev       Date:  2006-04       Impact factor: 26.132

6.  Development of an IP-Free Biotechnology Platform for Constitutive Production of HPV16 L1 Capsid Protein Using the Pichia pastoris PGK1 Promoter.

Authors:  F C Mariz; E C Coimbra; A L S Jesus; L M Nascimento; F A G Torres; A C Freitas
Journal:  Biomed Res Int       Date:  2015-05-18       Impact factor: 3.411

7.  Purification and Characterization of Botulinum Neurotoxin FA from a Genetically Modified Clostridium botulinum Strain.

Authors:  Sabine Pellett; William H Tepp; Marite Bradshaw; Suzanne R Kalb; Janet K Dykes; Guangyun Lin; Erin M Nawrocki; Christina L Pier; John R Barr; Susan E Maslanka; Eric A Johnson
Journal:  mSphere       Date:  2016-02-24       Impact factor: 4.389

8.  Tracking sources of Clostridium botulinum type E contamination in seal meat.

Authors:  Daniel Leclair; Jeffrey M Farber; Franco Pagotto; Sandy Suppa; Bill Doidge; John W Austin
Journal:  Int J Circumpolar Health       Date:  2017       Impact factor: 1.228

9.  Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A-hyper.

Authors:  Heather N'te Inzalaco; William H Tepp; Chase Fredrick; Marite Bradshaw; Eric A Johnson; Sabine Pellett
Journal:  mSphere       Date:  2021-08-04       Impact factor: 4.389
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.