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Literature DB >> 2450068

Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, C1, D, and E toxins and tetanus toxin.

K Tsuzuki¹, N Yokosawa, B Syuto, I Ohishi, N Fujii, K Kimura, K Oguma.

Abstract

The partial amino acid sequence of the light-chain (Lc) component of Clostridium botulinum type C1 toxin was determined. The sequence was quite similar to those of the other types of botulinum and tetanus toxins. Nine monoclonal antibodies against botulinum type E toxin were established by immunizing BALB/c mice with type E toxoid or its Lc component. Six antibodies reacted with the heavy-chain component and three reacted with the Lc component of the toxin. One of the latter three antibodies reacted with botulinum type B, C1, and D toxins and tetanus toxin, as well as botulinum type E toxin. This antibody recognized the Lc components of these toxins, indicating that there exists one common antigenic determinant on the Lc regions of these toxins.

Entities: Disease Species

Mesh：

Substances：

Year: 1988 PMID： 2450068 PMCID： PMC259387 DOI： 10.1128/iai.56.4.898-902.1988

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

17 in total

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Authors: N Yokosawa; K Tsuzuki; B Syuto; K Oguma
Journal: J Gen Microbiol Date: 1986-07

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Authors: K Yamamoto; I Ohishi; G Sakaguchi
Journal: Appl Environ Microbiol Date: 1979-02 Impact factor: 4.792

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Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

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Authors: S Nishi; H Yamazaki
Journal: Ann N Y Acad Sci Date: 1983 Impact factor: 5.691

5. Four different monoclonal antibodies against type C1 toxin of Clostridium botulinum.

Authors: K Oguma; T Agui; B Syuto; K Kimura; H Iida; S Kubo
Journal: Infect Immun Date: 1982-10 Impact factor: 3.441

6. Purification and characterization of two components of botulinum C2 toxin.

Authors: I Ohishi; M Iwasaki; G Sakaguchi
Journal: Infect Immun Date: 1980-12 Impact factor: 3.441

7. Isolation and molecular size of Clostridium botulinum type C toxin.

Authors: B Syuto; S Kubo
Journal: Appl Environ Microbiol Date: 1977-02 Impact factor: 4.792

8. Oral toxicities of Clostridium botulinum type A and B toxins from different strains.

Authors: I Ohishi
Journal: Infect Immun Date: 1984-02 Impact factor: 3.441

9. Separation and characterization of heavy and light chains from Clostridium botulinum type C toxin and their reconstitution.

Authors: B Syuto; S Kubo
Journal: J Biol Chem Date: 1981-04-25 Impact factor: 5.157

10. Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins.

Authors: U Eisel; W Jarausch; K Goretzki; A Henschen; J Engels; U Weller; M Hudel; E Habermann; H Niemann
Journal: EMBO J Date: 1986-10 Impact factor: 11.598

16 in total

1. Cloning and complete nucleotide sequence of the gene for the main component of hemagglutinin produced by Clostridium botulinum type C.

Authors: K Tsuzuki; K Kimura; N Fujii; N Yokosawa; T Indoh; T Murakami; K Oguma
Journal: Infect Immun Date: 1990-10 Impact factor: 3.441

2. Nucleotide sequence of Clostridium botulinum C1 neurotoxin.

Authors: D Hauser; M W Eklund; H Kurazono; T Binz; H Niemann; D M Gill; P Boquet; M R Popoff
Journal: Nucleic Acids Res Date: 1990-08-25 Impact factor: 16.971

3. Molecular diversity of neurotoxins from Clostridium botulinum type D strains.

Authors: K Moriishi; B Syuto; S Kubo; K Oguma
Journal: Infect Immun Date: 1989-09 Impact factor: 3.441

Review 4. Properties and use of botulinum toxin and other microbial neurotoxins in medicine.

Authors: E J Schantz; E A Johnson
Journal: Microbiol Rev Date: 1992-03

5. Sequence homology between tetanus and botulinum toxins detected by an antipeptide antibody.

Authors: J L Halpern; L A Smith; K B Seamon; K A Groover; W H Habig
Journal: Infect Immun Date: 1989-01 Impact factor: 3.441

6. Monoclonal antibody to type F Clostridium botulinum toxin.

Authors: J L Ferreira; M K Hamdy; S G McCay; F A Zapatka
Journal: Appl Environ Microbiol Date: 1990-03 Impact factor: 4.792

7. Epitope regions in the heavy chain of Clostridium botulinum type E neurotoxin recognized by monoclonal antibodies.

Authors: T Kubota; T Watanabe; N Yokosawa; K Tsuzuki; T Indoh; K Moriishi; K Sanda; Y Maki; K Inoue; N Fujii
Journal: Appl Environ Microbiol Date: 1997-04 Impact factor: 4.792

8. Cloning of the structural gene for Clostridium botulinum type C1 toxin and whole nucleotide sequence of its light chain component.

Authors: K Kimura; N Fujii; K Tsuzuki; T Murakami; T Indoh; N Yokosawa; K Oguma
Journal: Appl Environ Microbiol Date: 1991-04 Impact factor: 4.792

9. Pepsin fragmentation of botulinum type E neurotoxin: isolation and characterization of 112, 48, 46, and 16 kD fragments.

Authors: J A Giménez; B R DasGupta
Journal: J Protein Chem Date: 1992-06

10. Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents.

Authors: Jerry O Conway; Laura J Sherwood; M Thelma Collazo; John A Garza; Andrew Hayhurst
Journal: PLoS One Date: 2010-01-21 Impact factor: 3.240