Literature DB >> 351243

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A.

S Notermans, J Dufrenne, M Schothorst.   

Abstract

The enzyme linked immunosorbent assay using the so-called "double-sandwich technique" has been applied to determine botulinum toxin type A. By this assay, 50-100 mouse ip LD50 of toxin type A can be detected. No cross-reaction occurs with botulinum toxins of other types tested. In all probability this is due to the high specificity of the antiserum prepared against the toxic component of type A toxin.

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Year:  1978        PMID: 351243     DOI: 10.7883/yoken1952.31.81

Source DB:  PubMed          Journal:  Jpn J Med Sci Biol        ISSN: 0021-5112


  12 in total

1.  Comparison of mouse bioassay and immunoprecipitation assay for botulinum toxin antibodies.

Authors:  P A Hanna; J Jankovic; A Vincent
Journal:  J Neurol Neurosurg Psychiatry       Date:  1999-05       Impact factor: 10.154

Review 2.  Clostridium botulinum neurotoxin.

Authors:  H Sugiyama
Journal:  Microbiol Rev       Date:  1980-09

Review 3.  Laboratory diagnostics of botulism.

Authors:  Miia Lindström; Hannu Korkeala
Journal:  Clin Microbiol Rev       Date:  2006-04       Impact factor: 26.132

4.  Detection of Clostridium botulinum type A toxin by enzyme-linked immunosorbent assay with antibodies produced in immunologically tolerant animals.

Authors:  M Dezfulian; J G Bartlett
Journal:  J Clin Microbiol       Date:  1984-05       Impact factor: 5.948

5.  Colony immunoblot assay of botulinal toxin.

Authors:  M C Goodnough; B Hammer; H Sugiyama; E A Johnson
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

6.  Immunodiffusion method for detection of type A Clostridium botulinum.

Authors:  J L Ferreira; M K Hamdy; F A Zapatka; W O Hebert
Journal:  Appl Environ Microbiol       Date:  1981-12       Impact factor: 4.792

7.  Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin.

Authors:  S Notermans; J Dufrenne; S Kozaki
Journal:  Appl Environ Microbiol       Date:  1979-06       Impact factor: 4.792

8.  Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay.

Authors:  C Shone; P Wilton-Smith; N Appleton; P Hambleton; N Modi; S Gatley; J Melling
Journal:  Appl Environ Microbiol       Date:  1985-07       Impact factor: 4.792

9.  Detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay.

Authors:  G E Lewis; S S Kulinski; D W Reichard; J F Metzger
Journal:  Appl Environ Microbiol       Date:  1981-12       Impact factor: 4.792

10.  Noncorrelation between mouse toxicity and serologically assayed toxin in Clostridium botulinum type A culture fluids.

Authors:  M J Betley; H Sugiyama
Journal:  Appl Environ Microbiol       Date:  1979-08       Impact factor: 4.792

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